阳和汤含药血清对乳腺癌MCF-7细胞iNOS、COX-2及NF-κB表达的影响  

Effect of Yanghe decoction containing serum on expression of iNOS, COX-2 and NF-κB in breast cancer MCF-7 cells

作　　者：刘丽颖 窦建卫[3] 李高彪 杨硕 朱中博 黄芊[7,8] Liu Liying;Dou Jianwei;Li Gaobiao;Yang Shuo;Zhu Zhongbo;Huang Qian(Department of Surgery,Xi'an Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xi'an 710021,China;Department of Surgery,Xi'an Traditional Chinese Medicine Hospital,Xi'an 710021,China;School of Pharmacy,Xi'an Jiao Tong University Xi'an Shaanxi,Xi'an 710061,China;Department of ENT Xi'an Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xi'an 710021,China;Department of ENT,Xi'an Traditional Chinese Medicine Hospital,Xi'an 710021,China;Basic Medical College of Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Department of Science and Technology,Xi'an Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xi'an 710021, China;Department of Science and Technology,Xi'an Traditional Chinese Medicine Hospital Xi'an 710021, China)

机构地区：[1]陕西中医药大学西安附属医院外科,710021 [2]西安市中医医院外科,710021 [3]西安交通大学药学院,710061 [4]陕西中医药大学西安附属医院耳鼻喉科,710021 [5]西安市中医医院耳鼻喉科,710021 [6]甘肃中医药大学基础医学院,兰州730000 [7]陕西中医药大学西安附属医院科教科,710021 [8]西安市中医医院科教科,710021

出　　处：《国际中医中药杂志》2019年第2期173-176,共4页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81873311、81573983).

摘　　要：目的观察阳和汤含药血清干预对乳腺癌MCF-7细胞诱导型一氧化氮合酶(inducible nitric oxide synthasi, iNOS)、环氧合酶-2(cyclooxygenase-2, COX-2)及NF-κB的影响。方法用不同药物剂量(4.5、9、18 g/kg)制备的含药血清分别干预MCF-7人乳腺癌细胞,分为空白组、低剂量组、中剂量组、高剂量组。在24、48、72、96 h采用Real-time RT PCR法检测iNOS、COX-2 mRNA表达,Western-blot检测NF-κB蛋白表达。结果与空白组比较,干预24、48、72、96 h,低、中、高剂量组iNOS、COX-2 mRNA表达降低(P<0.05),NF-κB蛋白表达降低(P<0.05),且呈一定的浓度及时间依赖性,干预72 h后变化差异无统计学意义(P>0.05)。结论阳和汤可降低NF-κB蛋白及COX-2、iNOS mRNA表达水平。Objective To observe the changes of iNOS, COX-2 and NF-κB in breast cancer MCF-7 cells treated with Yanghe decoction containing serum. Methods The MCF-7 human breast cancer cells were divided into blank group, low dose group, middle dose group and high dose group (4.5, 9, 18 g/kg, respectively). Real-time RT-PCR was used to detect the mRNA expression of iNOS and COX-2 at 24, 48, 72 and 96 hours, and Western-blot was used to detect the expression of NF-κB protein. Results Compared with the blank group, the expression of iNOS and COX-2 mRNA in the low dose group, middle dose group and high dose group decreased significantly at 24, 48, 72 and 96 hours(P<0.05), the the expression of NF-κB protein in the low dose group, middle dose group and high dose group decreased significantly at 24, 48, 72 and 96 hours (P<0.05). So it reduced with the increase of drug concentration and time. There was no significant difference in 72 hours after intervention. Conclusions The Yanghe decoction could reduce the expression of NF-κB, and then reducing the related inflammatory factors COX-2 and iNOS.

关 键 词：MCF-7细胞 阳和汤 NF-ΚB 中药血清药理学 

分 类 号：R285.5[医药卫生—中药学]