地黄不同种质的遗传多样性和质量分析  

Genetic diversity and quality analysis of Rehmannia glutinosa in different germplasm

作　　者：石海霞 肖承鸿[1] 周涛[1] 江维克[1] 杨昌贵[1] 余意 张小波[3] 张成刚[1] SHI Hai-xia;XIAOCheng-hongx;ZHOU Tao;JIANG Wei-ke;YANG Chang-gui;YU Yi;ZHANG Xiao-bo;ZHANG Cheng-gang(Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China;Infinitus (China)Co,Ltd,Guangzhou 510553, China;Traditional Chinese Medicine Resource Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]无限极(中国)有限公司,广东广州510663 [3]中国中医科学院中药资源中心,北京100700

出　　处：《中国中药杂志》2018年第21期4210-4216,共7页China Journal of Chinese Materia Medica

基　　金：现代农业产业技术体系建设专项(CARS-21);贵州省国内一流建设学科项目(中药学)(GNYL[2017]008号);国家中医药管理局中医药公共卫生专项(国中医药规财[2014]146号);贵阳中医学院援黔专家团院士工作站(贵中医YQ合字[2017002]号)

摘　　要：该研究对地黄栽培种质的遗传多样性和药材质量进行综合评价,为筛选优良种质提供理论指导。采用SRAP分子标记分析21份地黄种质的遗传多样性,并结合HPLC测定其梓醇和毛蕊花糖苷的含量。结果表明,地黄种质的梓醇和毛蕊花糖苷的质量分数分别为2.393%~6.519%和0.063%~0.478%,其中种质14号、种质15号、种质16号和种质20号的梓醇及毛蕊花糖苷含量较高;10对引物共扩增出57条带,其中40条为多态性条带,各种质多态位点百分率为8.77%~54.39%,地黄总的Nei′s基因多样性指数(H)为0.374 1,Shannon′s多态性信息指数(I)为0.546 6;种质间遗传分化系数Gst与基因流Nm分别为0.608 8,0.321 3;基于遗传一致度,21份种质聚为2类。地黄种质遗传多样性水平为中等偏低水平;综合考虑梓醇、毛蕊花糖苷含量和遗传多样性水平,种质7和种质18可作为地黄栽培的优选材料;种质15和种质16可作为地黄种质的保存和品种选育对象。The study aims at evaluating genetic diversity and medicinal quality of cultivated germplasm in Rehmannia glutinosa, and providing theoretical guidance for screening excellent germplasm. The genetic diversity of 21 species of R. glutinosa were analyzed by SRAP molecular markers, and the catalpol and verbascoside was determined by HPLC. The mass fraction of catalpol and verbascoside in R. glutinosa germplasm were respectively in the range of 2.393%-6.519% and 0.063%-0.478%, the germplasm 14, 16, 15 and 20 germplasm, witch catalpol and verbascoside content was higher. A total of 57 bands were produced by 10 primer, among which 40 polymorphic bands were polymorphic bands, and the percentage of polymorphic loci was 8.77%-54.39%, the Nei′s genetic diversity index(H) was 0.374 1, Shannon′s polymorphism information index(I) was 0.546 6. Gst and gene flow Nm were 0.608 8 and 0.321 3, respectively. Based on the genetic uniformity, 21 species of germplasm were grouped into 2 categories. The genetic diversity level of R. glutinosa was medium low. The comprehensive consideration of the genetic diversity and the content inculde catalpol and verbascoside, germplasm 7 and germplasm 18 could be used as the preferred materials for the cultivation of reticulum. Germplasm 15 and 16 can be used as the preservation and breeding object of rhubarb germplasm.

关 键 词：地黄 SRAP 遗传多样性 质量评价 

分 类 号：S567.234[农业科学—中草药栽培]