基于β-AS基因多态性的金铁锁核心种质构建  

Construction of core collection of Psammosilene tunicoides based on polymorphism of β-AS gene

作　　者：吴利玲 高亚芳 钱子刚[1] 刘小莉[1] 李国栋[1,3] WU Li-ling;GAO Ya-fang;QIAN Zi-gang;LIU Xiao-li;LI Guo-dong(College of Traditional Chinese Medicine,Yunnan University of Traditional Chinese Medicine,Kunming 550500,China;College of Animal Science and Technology,Southwestern University,Chongqing 400715,China;Yunnan Key Laboratoor of Dai and Yi Medicines,Yunnan University of Traditional Chinese Medicine,Kunming 650500,China;Nanehong Central Hospital,Nanchong 637000,China)

机构地区：[1]云南中医学院中药学院,云南昆明650500 [2]西南大学动物科技学院,重庆400715 [3]云南中医学院云南省傣医药与彝医药重点实验室(筹备),云南昆明650500 [4]南充市中心医院,四川南充637000

出　　处：《中国中药杂志》2018年第21期4220-4225,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81560613);云南省科技厅-云南中医学院应用基础研究联合专项[2015FB205(-015)];云南省重点实验室培育计划项目(2017DG006)

摘　　要：金铁锁是"云南白药"的重要组分,为我国西南地区的特有二级濒危保护植物。其野生资源已不能满足医药生产的需求,亟需通过对其种质资源进行研究,构建金铁锁核心种质。为金铁锁种质改良、分子育种等奠定基础。该研究采用了关键酶基因(β-AS)作为核基因标记对金铁锁进行了谱系地理学研究。研究结果显示,在所有居群中,总共检测到36个单倍型,单倍型多样性(Hd=0.905)较高,居群间存在一定的遗传分化(GST=0.280)。AMOVA结果显示,金铁锁居群内遗传多样性较低(22.57%),居群间的遗传变异占77.43%。单倍型分布结合网状进化分析结果显示,Hap1为普通单倍型,且位于网状图的中心位置,可判断为祖先单倍型。Hap2,Hap4,Hap15,Hap16共享单倍型,剩余的为居群特有单倍型。在此基础上,为了最大限度的保存金铁锁的遗传多样性,依据药用植物核心种质构建的标准提出了金铁锁核心种质的构建策略。Psammosilene tunicoides is one of the main ingredients of the "Yunnan Baiyao". P. tunicoides is an endangered species included in the secondary protection list in China Plant Red Data Book as well as the endemic species in Southwest China. Its natural resources could not meet the needs of pharmaceutical production. Construction of core collection of P. tunicoides will lay the foundation for germplasm improvement and molecular breeding. The sequence variation of the key enzymes gene locus(β-AS) were carried out to survey the population structure and population history of the species. Among the 11 populations across its geographical range, 36 haplotypes were identified. The levels of haplotype diversity(Hd=0.905) were high, while the levels of population differentiation(GST=0.280) were low. Analysisof molecular variance(AMOVA) indicated that a significantly greater proportion of total genetic variationpartitioned among populations thanwithin populations(values of 77.43% and 22.57%, respectively). These results in combination with the star-like phylogenetic network analysis indicate that Hap1 as an ancestral haplotypewas shared in four populations, Hap2, Hap4, Hap15 and Hap16 are occurred in two populations, the remains as private haplotype only distributed in single population. The strategy of core collection was constructed in order to maximumpreserve genetic diversity of P. tunicoides.

关 键 词：金铁锁 β-AS基因 谱系地理学 核心种质 

分 类 号：S567.239[农业科学—中草药栽培]