黑白花牛INHA基因多态性与超数排卵性状关系  

Study on Polymorphism and Superovulation Trait Relationship of INHA Gene in Holstein

作　　者：罗布[1] 朱彦宾[2] 巴桑旺堆 平措占堆[2] 次仁卓玛 尼玛[2] 梁春芳[5] 德吉曲宗 Luo Bu;Zhu Yanbin;Basang Wangdui;Pingcuo Zhandui;Ciren Zhuoma;Ni Ma;Liang Chunfang;Deji Quzong(Institute of Aegetables,College of Agriculture and Animal Husbandry,Lhasa,850000;Institute of.Animal Husbandry and Veterinary Medicine, College of Agriculture and Animal Husbandry,Lhasa,850009;The Tibet Autonomous Region of Agriculture and Animal Husbandry Research Institute of Agricultural Resources and the Environment,Lhasa,850000;Sangzhu Zi District of Shigatse City Animal Husbandry and Veterinary Station, Shigatse,850000;Tibet Autonomous Region College of Agriculture and Animal Husbandry,Lhasa,850000)

机构地区：[1]西藏自治区农牧科学院蔬菜研究所,拉萨850000 [2]西藏自治区农牧科学院畜牧兽医研究所,拉萨850009 [3]西藏自治区农牧科学院农业资源与环境研究所,拉萨850000 [4]日喀则市桑珠孜区畜牧兽医站,日喀则850000 [5]西藏自治区农牧科学院,拉萨850000

出　　处：《基因组学与应用生物学》2019年第2期511-515,共5页Genomics and Applied Biology

基　　金：自治区项目"良种奶牛引进与冻精生产制作应用技术研究";自治区自然基金"提高奶牛产奶量的营养调控技术研究"(2016ZR-15-43)共同资助

摘　　要：为了研究黑白花牛抑制素α基因(INHA)的遗传多样性和超数排卵效果的关联分析,找到一种能够作为黑白花牛超数排卵预测的候选基因,本试验以50头黑白花牛为实验材料,对其进行了超排处理并且采集了黑白花牛的血液样本提取基因组。采用PCR-SSCP技术,在INHA基因的第一外显子和第二外显子各设计了一对引物进行基因型检测,然后与超排性状进行关联分析。结果表明:黑白花牛INHA基因的177 bp处(+1为转录起始位点)存在一个A>G突变的多态性位点,AA和AG基因型的频率分别为0.62和0.38。与超排性状关联分析,结果显示AG基因型黑白花牛的排卵数和鲜胚数均显著多于AA基因型(p<0.05),这说明黑白花牛个体的超排性状可能与其本身的INHA基因的遗传特性有关。In order to study the correlation analysis of genetic diversity and the effect of superovulation of inhibin alpha gene(INHA) in Holstein and to be able to find a super ovulation prediction candidate gene of Holstein, we took50 head of Holsteins as the experimental materials, carried on the superovulation and collected Holstein blood samples to extract genome. A pair of primers were designed for genotype detection in the first exon and the second exon of INHA gene by PCR-SSCP, which were then made correlation analysis for superovulation traits. The results showed that at the 177 bp of INHA gene(+1 transcription initiation site) of Holstein, there was a polymorphic site of A>G mutation, and the genotype frequencies of AA and AG were 0.62 and 0.38, respectively. The results of superovulation association analysis showed that the number of ovulation and fresh embryo of AG genotypes of Holstein were significantly higher than that of AA genotype(p<0.05), which indicated the superovulation traits of Holstein individuals might be related to the genetic characteristics of the INHA gene itself.

关 键 词：黑白花牛 抑制素α(INHA) 多态性 超数排卵 

分 类 号：S823[农业科学—畜牧学]