OAZ在果蝇大脑发育中的功能研究  

Function Study of OLE1/EBF-associated Zinc Finger Protein(OAZ) in Drosophila Brain Development

作　　者：黄燕怡 袁伏琪 Huang Yanyi;Yuan Fuqi(College of Biology,Hunan University,Changsha,410082)

机构地区：[1]湖南大学生物学院,长沙410082

出　　处：《基因组学与应用生物学》2019年第2期556-562,共7页Genomics and Applied Biology

基　　金：国家重大基础研究计划项目<脑发育的分子调控机制>资助

摘　　要：OAZ基因编码在进化上保守的C2H2型O/E相关锌指蛋白,参与基因转录的调控。TGF-β信号传导通路中OAZ通过与SMAD4/R-SMAD结合形成转录复合物发挥作用;Olf1/EBF作为转录因子与OAZ相互作用来调控嗅觉上皮细胞和B淋巴细胞的分化。此外,OAZ是JAK/STAT信号通路的下游候选靶基因。但是迄今为止OAZ在果蝇大脑发育的功能还没有研究。果蝇大脑视叶作为一个相对简易操作的模型为我们揭示早期神经干细胞的增殖和转化机制创造了条件,为加快理解哺乳动物早期神经发生过程以及进一步开展神经干细胞治疗提供可能。本研究通过RNA干扰来研究OAZ在果蝇大脑发育中的作用。我们的初步实验结果表明,OAZ对果蝇大脑视叶神经上皮细胞的维持可能不是必需的,OAZ对果蝇大脑视叶神经板和脑髓神经节的发育也可能不是必需的。The OAZ gene encoded an evolutionarily conserved C2 H2 O/E-related zinc finger protein, which was involved in the regulation of gene transcription. In the TGF-β signaling pathway, OAZ interacted with SMAD4/R-SMAD to form transcriptional complexes;Olf1/EBF acted as a transcription factor to interact with OAZ to regulate the differentiation of olfactory epithelial cells and B lymphocytes. In addition, OAZ was a candidate target gene of the JAK/STAT signaling pathway. However, the role of OAZ in Drosophila brain development has not been investigated. The Drosophila larval optic lobe as a model, which was relatively simple to operate created a condition for us to reveal the multiplication and transfer mechanism of early neural stem cells. And it was possible to accelerate the understanding of early neurogenesis in mammals and to further develop neural stem cell therapy. Here the role of the OAZ gene in the regulation of neural stem cells was examined through knockdown of OAZ m RNA. Our data suggested that OAZ might not be necessary to maintain the neuroepithelia in Drosophila optic lobes, and OAZ might not be necessary for the development of lamina and medulla, two visual processing centers in the brain.

关 键 词：果蝇 大脑视叶 神经上皮干细胞 OAZ 

分 类 号：Q963[生物学—昆虫学]