蝴蝶兰PhTSJT1基因的克隆及在低温胁迫下的表达分析  

Cloning of PhTSJT1 Gene from Phalaenopsis and Its Expression under Low Temperature Stress

作　　者：袁秀云[1] 许申平[1] 王默霏[1] 雷志华 崔波[1] Yuan Xiuyun;Xu Shenping;Wang Mofei;Lei Zhihua;Cui Bo(Institute of Bioengineering,Zhengzhou Normal University,Zhengzhou,450044;School of Life Sciences,Zhengzhou Normal University, Zhengzhou,450044)

机构地区：[1]郑州师范学院生物工程研究所,郑州450044 [2]郑州师范学院生命科学学院,郑州450044

出　　处：《基因组学与应用生物学》2019年第2期707-713,共7页Genomics and Applied Biology

摘　　要：本研究从蝴蝶兰叶片中克隆了茎部特异基因Ph TSJT1的全长序列(GenBank登录号为MF797883),并分析了其在不同组织及低温胁迫下的表达特性。结果表明,Ph TSJT1基因全长994 bp,编码239个氨基酸,属于Class-Ⅱ谷氨酰胺酰胺基转移酶超家族成员;同源性分析表明,该蛋白与多种植物的茎部特异蛋白和铝诱导蛋白有较高的同源性,进化上与小兰屿蝴蝶兰的茎部特异蛋白亲缘关系最近;该基因在营养器官中表达水平较高,在花器官中表达水平较低;13℃/8℃(昼/夜)的低温胁迫抑制PhTSJT1基因的转录表达,并随着低温胁迫时间的延长,Ph TSJT1基因的表达水平逐渐降低,在温度恢复正常时其表达水平升高;4℃冷胁迫低温条件下,PhTSJT1基因在处理1 h时,表达水平升高,处理8 h时表达水平最高,16 h后表达水平逐渐降低。由此推测,PhTSJT1参与4℃冷胁迫的分子调控。本研究不但有助于理解热带亚热带植物的耐冷机制,也为蝴蝶兰新品种的遗传改良提供帮助。The study cloned the full length sequence of stem specific gene PhTSJT1(GenBank accession number:MF797883) from the leaf of Phalaenopsis and analyzed the expression features under different issues and low temperature stress. The results showed the full-length sequence of PhTSJT1 gene was 994 bp, encoding 239 amino acids, which belonged to the superfamily of Class-Ⅱ glutamine transferases. The PhTSJT1 protein was highly homologous to stem-specific proteins and aluminum induced proteins from different species. Phylogenetic analysis indicated that the protein was closely related to stem-specific protein from Phalaenopsis equestris. The expression levels of PhTSJT1 gene were higher in vegetative organs than that of in floral organs. The transcriptional expression level of PhTSJT1 gene was inhibited with low temperature treatment of 13℃/8℃(day/night). With the extension of low-temperature stress time, the expression level of PhTSJT1 gene gradually decreased, then increased after the recovering to normal temperature. Under the cold stress of 4℃, the expressions of PhTSJT1 gene were increased at 1 h, and achieved the highest level at 8 h, of which the expression level was decreased gradually after treatment for 16 h. The results suggested that Ph TSJT1 participated in the molecular regulation of 4℃ cold stress.This study would be beneficial to understand the mechanism of cold tolerance in tropical and subtropical plant, as well as provide an aid for genetic improvement of new species in Phalaenopsis.

关 键 词：蝴蝶兰 低温胁迫 PhTSJT1基因 铝诱导蛋白 基因表达 

分 类 号：S682.31[农业科学—观赏园艺] Q943.2[农业科学—园艺学]