高效液相色谱测法定防晒霜中双-乙基己氧苯酚 甲氧苯基三嗪的含量  

Determination of bis-ethylhexyloxyphenol methoxyphenyl triazine in sun lotions by HPLC

作　　者：徐文峰[1] 金鹏飞[1] 徐硕[1] 邝咏梅[1] 何笑荣[1] XU Wen-feng;JIN Peng-fei;XU Shuo;KUANG Yong-mei;HE Xiao-rong(Department of Pharmacy,Beijing Hospital,National Center of Gerontology,Key Laboratory of Clinical Drug Risk Assessment and Individual Application,Beijing 100730)

机构地区：[1]北京医院药学部国家老年医学中心药物临床风险与个体化应用评价北京市重点实验室,北京100730

出　　处：《中南药学》2019年第2期240-242,共3页Central South Pharmacy

基　　金：北京医院科技新星项目(No.BJ-2016-039)

摘　　要：目的应用高效液相色谱建立防晒霜中双-乙基己氧苯酚甲氧苯基三嗪的含量测定方法。方法采用Alltima C18色谱柱(150 mm×4.6 mm,5μm),5%四氢呋喃乙腈溶液为流动相,流速为2.5mL·min^(-1),检测波长为340 nm。结果制剂辅料不干扰双-乙基己氧苯酚甲氧苯基三嗪的测定,双-乙基己氧苯酚甲氧苯基三嗪在10.1～201.4μg·m^L^(-1)与峰面积线性关系良好,相关系数为0.9997。精密度和稳定性考察RSD均小于2%,重复性与回收试验RSD均小于3%。结论该方法简便,准确,灵敏度高,专属性强,可用于测定防晒霜中双-乙基己氧苯酚甲氧苯基三嗪的含量。Objective To establish a high performance liquid chromatography(HPLC) method to determine bisethylhexyloxyphenol methoxyphenyl triazine in sun lotion. Methods An Alltima C18 column(150 mm×4.6 mm, 5 μm) was used for the separation with mobile phase of 5% tetrahydrofuran acetonitrile solution at a flow rate of 2.5 mL·min^-1. The detection wavelength was 340 nm. Results The excipients did not interfere with the analysis of bis-ethylhexyloxyphenol methoxyphenyl triazine. The linearity of bis-ethylhexyloxyphenol methoxyphenyl triazine was 10.1-201.4 μg·m L^-1 with the correlation coefficient 0.9997. The precision and stability were satisfactory with RSDs below 2%. RSDs of repetitive tests and the spiked recoveries were below 3%. Conclusion This method is simple and accurate with high sensitivity and specificity, and suitable for the determination of bis-ethylhexyloxyphenol methoxyphenyl triazine in sun lotion.

关 键 词：高效液相色谱法 双-乙基己氧苯酚甲氧苯基三嗪 防晒霜 含量测定 

分 类 号：R917[医药卫生—药物分析学] R927[医药卫生—药学]