Triazole Ureas Covalently Bind to Strigolactone Receptor and Antagonize Strigolactone Responses  

作　　者：Hidemitsu Nakamura Kei Hirabayashi Takuya Miyakawa Ko Kikuzato Wenqian Hu Yuqun Xu Kai Jiang Ikuo Takahashi Ruri Niiyama Naoshi Dohmae Masaru Tanokura Tadao Asami 

机构地区：[1]Graduate School of Agricultural and Life Sciences,The University of Tokyo,1-1-1 Yayoi,Bunkyo-ku,Tokyo 113-8657,Japan [2]Biomolecu]ar Characterization Unit,RIKEN Center for Sustainable Resource Science,Wako,Saitama,Japan [3]Department of Biochemistry,Faculty of Science,King Abdulaziz University,P.O.Box 80203,Jeddah 21589,Saudi Arabia

出　　处：《Molecular Plant》2019年第1期44-58,共15页分子植物（英文版）

摘　　要：Strigolactones,a class of plant hormones with multiple functions,mediate plant-plant and plantmicroorganism communications in the rhizosphere.In this study,we developed potent strigolactone antagonists,which covalently bindto the strigolactone receptor D14,by preparing an array of triazole urea compounds.Using yeast two-hybrid and rice-tillering assays,we identified a triazole urea compound KK094 as a potent inhibitor of strigolactone receptors.Liquid chromatography-tandem mass spectrometry analysis and X-ray crystallography revealed that KK094 was hydrolyzed by D14,and that a reaction product of this degradation covalently binds to the Ser residue of the catalytic triad of D14.Furthermore,we identified two triazole urea compounds KK052 and KK073,whose effects on D14-D53/D14-SLR1 complex formation were opposite due to the absence (KK052)or presence (KK073)of a trifluoromethyl group on their phenyl ring.These results demonstrate that triazole urea compounds are potentially powerful tools for agricultural application and may be useful for the elucidation of the complicated mechanism underlying strigolactone perception.

关 键 词：STRIGOLACTONE COVALENT ANTAGONIST TRIAZOLE urea crystal structure 

分 类 号：Q[生物学]