Regulation of Root-Knot Nematode Behavior by Seed-Coat Mucilage-Derived Attractants  

作　　者：Allen Yi-Lun Tsai Takumi Higaki Chinh-Nghia Nguyen Laetitia Perfus-Barbeoch Bruno Favery Shinichiro Sawa 

机构地区：[1]Graduate School of Science &Technology,Kumamoto University,2-39-1 Kurokami,Chuo-ku,Kumamoto 860-8555,Japan [2]Graduate School of Frontier Sciences,The University of Tokyo,5-1-5 Kashiwanoha,Kashtwa-shi,Chiba 277-8562,Japan [3]INRA,Universite Cote d'Azur,CNRS,UMR 1355-7254 Institut Sophia Agrobiotech,06900 Sophia AntipolJs,France [4]present address:Internattonal Research Organization for Advanced Science &Technology,Kumamoto University,2-39-1 Kurokami,Chuo-ku,Kumamoto 860-8555,Japan [5]Present address:School of Biotechnology and Food Technology,Hanoi University of Science and Technology,10 000 Hanoi,Vietnam

出　　处：《Molecular Plant》2019年第1期99-112,共14页分子植物（英文版）

摘　　要：Seed exudates influence the behavior of soil organisms,but howthis occurs remains unclear,particularly for multicellular animals.Here we show that compounds associated with Arabidopsis seed-coat mucilage regulate the behavior of soil-borne animals,specifically root-knot nematodes (RKNs).Infective RKN J2 larvae actively travel toward Arabidopsis seeds through chemotaxis.Analysis of Arabidopsis mucilage mutants demonstrated that the attraction of RKNs toArabidopsis seeds requires the synthesis and extrusion of.seed-coat mucilage.Extracted mucilage alone is not sufficient to attract RKNs,but seed-surface carbohydrates and proteins are required for this process.These findings suggest that the RKN chemoattractant is synthesized de novo upon mucilage extrusion but may be highly unstable.RKNs attracted by thismucilage-dependent mechanism can infect the emerging seedling.However,the attraction signal from seedling roots likely acts independently of the seed-coat signal and may mask the attraction to seed-coat mucilage after germination.Multiple RKN species are attracted byArabidopsis seeds,suggesting that this mechanism is conserved in RKNs.These findings indicate that seed exudate can regulate the behavior of multicellular animals and highlight the potential roles of seed-coat mucilage in biotic interactions with soil microorganisms.

关 键 词：CHEMOTAXIS seed-coat mucilage ROOT-KNOT nematode PLANT-PATHOGEN interaction 

分 类 号：Q[生物学]