四川部分区域鸽源沙门菌耐药表型及耐药基因检测  

Detection of antibiotic resistance and resistance genes of Salmonella in pigeons in some areas in Sichuan

作　　者：张跃东 罗薇[1] 张焕容[1] 任玉鹏[1] 葛润洲 杨劲松 ZHANG Yuedong;LUO Wei;ZHANG Huanrong;REN Yupeng;GE Runzhou;YANG Jinsong(College of Life Science and Technology,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《畜牧与兽医》2019年第2期52-58,共7页Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划项目(2017YFD0501101);四川省科技计划项目(2017NFP0046)

摘　　要：为了解四川省成都市及周边区域鸽源沙门菌的耐药表型及耐药基因携带情况,通过常规细菌分离鉴定并结合PCR方法,获得42株鸽源沙门菌,采用K-B纸片法,选取20种抗生素,对42株鸽源沙门菌进行药敏试验,并结合PCR方法对其耐药基因携带情况进行检测。结果显示:受检菌对链霉素、四环素、多西环素耐药率为88. 1%;对阿莫西林耐药率为83. 3%;对头孢拉定、氨苄西林、萘啶酸的耐药率为81. 0%。42株沙门菌皆有不同程度的多重耐药性,其中33株同时耐4种以上抗生素,占检测菌株的78. 6%(33/42),主要的耐药谱为CTX-CRO-FLO-CPIOFL-AKN。42株沙门菌喹诺酮类耐药基因aac (6')-Ib-cr检出率最高,为88. 09%(37/32);氨基糖苷类耐药基因aadA1、aph (3)Ⅱ、aac(3)Ⅱ检出率分别为80. 95%(34/42)、76. 19%(32/42)、16. 67%(7/42);β-内酰胺类耐药基因blaTEM-1、bla CMY-2检出率分别为42. 86%(18/42)、19. 04%(8/42);四环素类耐药基因tet M、tet A、tet B检出率分别为40. 4%(17/42)、38. 09%(16/42)、9. 52%(4/42);磺胺类耐药基因sul2检出率为80. 95%(34/42);喹诺酮类耐药基因qnrA检出率为38. 09%(16/42)。结果表明:四川省成都市及周边区域鸽源沙门菌存在普遍耐药,且多重耐药较为严重,耐药基因携带率高。This study was to investigate the antibiotic resistance and resistance genes of Salmonella in pigeons in Chengdu and its surrounding areas in Sichuan Province. A total of 42 strains of salmonella were isolated from pigeons using normal method for bacterial isolation combined with PCR. The antimicrobial susceptibility of the Salmonella isolates was examined by the K-B method. Then,PCR was used to detect the resistance genes of the isolates. The results showed that the resistance rates of the 42 isolated strains to streptomycin,tetracycline,doxycycline were high at 88. 1%. Their resistance to amoxicillin was 83. 3%,and to cefradine,ampicillin and nalidixic acid were all 81. 0%. All the 42 strains of Salmonella were of multidrug resistance at different degrees,with 33 of them being resistant to more than four antibiotics and accounting for 78. 6%( 33/42) of the total. The main resistant profile was CTX-CRO-FLO-CPI-OFL-AKN. Detection of resistance determinants revealed that aac( 6’)-Ib-cr was the most prevalent gene. Three kinds of aminoglycoside resistance genes aad A1( 80. 95%,34/42),aph( 3) Ⅱ( 76. 19%,32/42),and aac( 3)( 16. 67%,7/42) were all detected in isolates. The detected rates of bla TEM-1 and bla CMY-2 were 42. 86%( 18/42) and 19. 04%( 8/42),respectively. Among the four tetracycline resistance genes,tet M( 40. 4%,17/42),tet A( 38. 09%,16/42) and tet B( 9. 52%,4/42) were the most common resistance genes of the isolated strains. The detection rates of sul2 and qnr A were 80. 95%( 34/42) and 38. 09%( 16/42),respectively. These results indicated that the resistance rate of the isolated strains from pigeons in the investigated areas in Sichuan Province was high with wide a drug resistant spectrum,and the prevalence of drug resistant genes was very common. The present data provide a theoretical basis for treatment of Salmonella in pigeons,and are of significane for public health.

关 键 词：鸽 沙门菌 耐药性 耐药基因 四川 

分 类 号：S836[农业科学—畜牧学]