藏系绵羊mtDNA和Y染色体遗传多样性的检测与系统进化关系的分析  

Genetic diversity and phylogenetic evolution of mitochondrial DNA and Y-chromosome in Tibetan sheep

作　　者：董泽生 高雪 吴燕华 李蕾蕾 付弘赟 张瑞娜 贾功雪 DONG Zesheng;GAO Xue;WU Yanhua;LI Leilei;FU Hongyun;ZHANG Ruina;JIA Gongxue(Ledu Animal Husbandry and Veterinary Station of Haidong City,Haidong 810700,China;Northwest Institute of Plateau Biology,Chinese Academy of Seienees,Xining 810001,China;Ministry of Agrieuhure and Rural Affairs of the People's Republic of China,Beijing 100125,China;Qinghai Animal Husbandry Station,Xining 810008,China)

机构地区：[1]海东市乐都区畜牧兽医站,青海海东810700 [2]中国科学院西北高原生物研究所,西宁810001 [3]中华人民共和国农业农村部,北京100125 [4]青海省畜牧总站,西宁810008

出　　处：《黑龙江畜牧兽医》2019年第4期45-47,51,179,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家重点研发计划项目(2016YFC0501805);青海省重点研发与转化计划项目(2017-NK-114);青海省自然科学基金项目(2017-ZJ-915Q)

摘　　要：为了评估青海地区藏系绵羊各类群的遗传结构与分子进化关系,试验选择青海地区四个典型的地理生态类群(山谷型、欧拉型、高原型和扎什加型)藏羊,提取血液基因组DNA后,分别对线粒体D-loop区序列和Y染色体Sry基因序列进行特异性扩增并测序。结果表明:D-loop序列的核苷酸多样性为0.031±0.004,单倍型多样性为0.994±0.006;而Sry序列的核苷酸多样性为0.046±0.005,单倍体多样性为0.861±0.044。说明高原型藏羊在母系遗传上更接近扎什加型藏羊,而在父系遗传上扎什加型藏羊与其他三个类群间的隔离较为明显,三个类群在父系遗传上的基因交流更为频繁。To evaluate the genetic structure and phylogenic relationships of Tibetan sheep breeds in Qinghai Plateau, the study obtained blood samples from four distributed geographic ecotype populations(Valley Type,Euler Type, Plateau Type and Zhashijia Type). The displacement-loop(D-loop) region on mitochondrial DNA(mtDNA) and the sex determining region Y(Sry) gene sequences on Y chromosome were cloned and sequenced. The results showed that the nucleotide diversity and haplotype diversity were 0.031±0.004 and 0.994±0.006 based on D-loop sequences, and 0.046±0.005 and 0.861±0.044 based on Sry sequences, respectively. The phylogenetic analysis revealed that the Plateau type populations were closely related to Zhashijia Type populations maternally, while Zhashijia Type populations exhibited obvious isolation with other three populations paternally. In contrast, the gene communication among three populations more frequently in the patrilineal genetics.

关 键 词：藏系绵羊 线粒体DNA Y染色体 系统发育 遗传多样性 遗传距离 

分 类 号：S826.83[农业科学—畜牧学] S813.1[农业科学—畜牧兽医]