高原牦牛肺脏组织诱导型一氧化氮合酶基因表达分析  

The expression analysis of inducible nitric oxide synthase gene from Plateau yak lung

作　　者：李凤 张晶晶[1] 俞红贤[1] 荆海霞[1] 李莉[1] 魏青[1] 梁林[1] 王昕玥 张勤文[1] LI Feng;ZHANG Jingjing;YU Hongxian;JING Haixia;LI Li;WEI Qing;LIANG Lin;WANG Xinyue;ZHANG Qinwen(Veterinary Medicine Department of Agricultural and Animal Husbandry College,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农牧学院动物医学系,西宁810016

出　　处：《黑龙江畜牧兽医》2019年第3期135-138,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31660699)

摘　　要：为了阐明高原牦牛诱导型一氧化氮合酶(iNOS)mRNA的表达对高原牦牛肺脏组织低氧适应作用的影响,试验采用实时荧光定量PCR方法,对不同品种、不同海拔高度的牦牛iNOS的mRNA相对表达量进行相关分析。结果表明:iNOS在转录水平上总体表现为随海拔的升高而升高的趋势,且父本来自海拔5 000 m以上的大通牦牛肺脏组织的iNOS mRNA相对表达量最高。说明牦牛iNOS mRNA相对表达量与海拔和品种均有一定的相关性。In order to investigate the function about expression of iNOS mRNA in the Plateau yak ( Bos mutus) pulmonary hypoxia adaptation, the Plateau yaks from different elevation were selected as the research object. The quantitative real-time PCR was conducted to quantify relative abundances of iNOS transcripts in the lung tissues of Plateau yak at different altitudes. The results revealed that the transcription level of induced nitric oxide synthase increased with altitudes. The relative expression of iNOS mRNA of Datong yaks which male parent were at 5 000 meters above sea level was the highest.The results indicate that the transcription level of iNOS mRNA have some relevance to the altitudes and varieties.

关 键 词：高原牦牛 诱导型一氧化氮合酶 低氧适应 实时荧光定量PCR 基因相对表达量 

分 类 号：S823.85[农业科学—畜牧学] Q786[农业科学—畜牧兽医]