吸水链霉菌FC-904发酵代谢产物29-O-去甲基雷帕霉素的分离和结构鉴定  

Isolation and identification of 29-O-desmethylrapamycin produced by sirolimus producing strain FC-904

作　　者：杨国新[1] 陈夏琴[1] 余辉[1] 黄洪祥[1] 金东伟[1] 程元荣[1] 黄捷[1] Yang Guo-xin;Chen Xia-qin;Yu Hui;Huang Hong-xiang;Jin Dong-wei;Cheng Yuan-rong;Huang jie(Fujian Provincial Key Laboratory of Screening for Novel Microbial Products,Fujian Institute of Microbiology,Fuzhou 350007)

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福州350007

出　　处：《中国抗生素杂志》2019年第2期208-213,共6页Chinese Journal of Antibiotics

基　　金：福州市科技项目(No.2017-G-92)

摘　　要：目的从西罗莫司产生菌吸水链霉菌FC904的发酵液中分离纯化代谢产物FIM-R85,鉴定其化学结构。方法采用硅胶柱层析和制备液相分离纯化获得FIM-R85,进行理化性质以及NMR、UV、IR和HRMS等波谱分析,鉴定其化学结构。结果纯化获得HPLC纯度为98.7%的FIM-R85,其为西罗莫司类似物,与29-O-去甲基雷帕霉素同质。结论 29-O-去甲基雷帕霉素为西罗莫司产生菌吸水链霉菌FC904的发酵代谢产物之一。Objective To isolate and identify FIM-R85, a metabolite from the broth of sirolimus producing strain Streptomyces hygroscopicus FC904. Methods FIM-R85 was separated by silica gel chromatography and preparative liquid chromatography. Its chemical structure was determined by physicochemical properties and spectral analysis including NMR, UV, IR as well as HRMS. Results The HPLC purity of FIM-R85 was 98.7% and it was an analogue of sirolimus, identical with 29-O-desmethylrapamycin. Conclusion 29-O-desmethylrapamycin was one of the metabolites of sirolimus producing strain Streptomyces hygroscopicus FC904.

关 键 词：西罗莫司 29-O-去甲基雷帕霉素 吸水链霉菌FC904 分离纯化 结构鉴定 

分 类 号：R979.5[医药卫生—药品]