燃煤污染型砷中毒患者外周血Foxp3、TGF-β1、IL-2的表达差异及意义  

Differential expression and significance of Foxp3, TGF-β1 and IL-2 in peripheral blood of patients with coal-burning arsenic poisoning

作　　者：方晓琳 夏仕青 朱凯[1] 董令[1] 邹忠兰 刘永莲[1] 王大朋[1] 张爱华[1] Fang Xiaolin;Xia Shiqing;Zhu Kai;Dong Ling;Zou Zhonglan;Liu Yonglian;Wang Dapeng;Zhang Aihua(Key Laboratory for Environmental Pollution Monitoring and Disease Control,Ministry of Education,Department of Toxicology,Guizhou Medical University,Guiyang 550025,China)

机构地区：[1]贵州医科大学环境污染与疾病监控教育部重点实验室卫生毒理学教研室,贵阳550025

出　　处：《中华地方病学杂志》2019年第2期91-95,共5页Chinese Journal of Endemiology

基　　金：国家自然科学基金(81430077、81730089).

摘　　要：目的观察燃煤污染型砷中毒患者外周血转录因子叉头/翼状螺旋转录因子3(Foxp3)、免疫因子转化生长因子-β1(TGF-β1)、T淋巴细胞活化相关因子白细胞介素2(IL-2)的表达水平变化,分析砷暴露对机体免疫功能的影响。方法采用病例-对照研究方法,以贵州省黔西南州雨樟燃煤污染型砷中毒病区为调查点,参照《地方性砷中毒诊断》(WS/T211-2015)标准,选择经临床复查确认的149例[男性94例、女性55例,年龄为(50.69±6.14)岁]砷中毒患者为病例组,根据皮肤损害将其分为轻度砷中毒组(39例)、中度砷中毒组(54例)和重度砷中毒组(56例);另选择41例[男性12例、女性29例,年龄为(45.76±7.88)岁]距病区约12km的非砷暴露村居民作为对照组。在知情同意下采集研究对象晨尿及外周血,采用电感耦合等离子体质谱(ICP-MS)检测尿砷含量,并经尿肌酐(Cr)校正;采用实时荧光定量PCR方法检测外周血调节性T细胞(Treg细胞)特异转录因子Foxp3基因的表达水平;采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清Treg细胞相关免疫因子TGF-β1及IL-2的含量。结果各砷中毒组患者尿砷含量[中位数(四分位数):29.13(19.75~54.50)、31.81(17.52~53.31)、30.51(18.35~45.76)μg/gCr]高于对照组[21.62(17.65~28.44)μg/gCr,P均<0.05]。各砷中毒组患者外周血Foxp3mRNA表达水平[中位数(四分位数):0.58(0.17~1.27)、0.32(0.17~0.61)、0.33(0.13~0.62)]显著低于对照组[0.87(0.64~1.50),P均<0.05];与轻度砷中毒组比较,中、重度砷中毒组患者外周血Foxp3mRNA表达水平降低(P均<0.05)。各砷中毒组患者血清TGF-β1含量[(13.14±5.19)、(12.85±5.51)、(12.78±4.95)μg/L]高于对照组[(3.90±1.53)μg/L,P均<0.05]。各砷中毒组患者血清IL-2含量[(9.85±5.38)、(11.64±6.40)、(12.27±6.19)ng/L]低于对照组[(34.30±4.84)ng/L,P均<0.05],重度砷中毒组患者血清IL-2含量高于轻度砷中毒组(P<0.05)。结论砷暴露可致患者外�Objective To investigate the expression of transcription factor forkhead/winged helix transcription factor 3 (Foxp3), immune factor transforming growth factor-beta 1 (TGF-β1), and T-lymphocyte activation related factor interleukin-2 (IL-2) in peripheral blood of patients with coal-burning arsenic poisoning, and to analyze the effects of arsenic exposure on immune function. Methods A case-control study was conducted to investigate 149 cases [94 males and 55 females, (50.69 ± 6.14) years old] of arsenic poisoning in Yuzhang coal-burning arsenic poisoning area, southwestern Guizhou Province, and the cases were diagnosed based on the "Diagnosis of Endemic Arsenicosis" (WS/T 211-2015) and confirmed by clinical review. According to skin damage, the patients were divided into mild arsenic poisoning group (39 cases), moderate arsenic poisoning group (54 cases) and severe arsenic poisoning group (56 cases);and 41 cases [12 males and 29 females, (45.76 ± 7.88) years old] of non-arsenic exposed residents from 12 km of Yuzhang coal-burning area were selected as control group. Morning urine and peripheral blood samples were collected with informed consent. Urine arsenic content was measured by inductively coupled plasma mass spectrometry (ICP-MS). Urine arsenic was corrected by creatinine (Cr). Detection of regulatory T cell (Treg)-specific transcription factor Foxp3 gene expression in human peripheral blood was done by real-time fluorescence quantitative PCR, and the levels of Treg-related immune factor TGF-β1 and IL-2 in serum were detected by enzyme linked immunosorbent assay (ELISA). Results The urinary arsenic contents [median (quartile): 29.13 (19.75-54.50), 31.81 (17.52-53.31), 30.51 (18.35-45.76) μg/g Cr] in each arsenic poisoning group were higher than that in the control group [21.62 (17.65-28.44) μg/g Cr, P < 0.05]. The expression levels of Foxp3 mRNA in peripheral blood of each arsenic poisoning group [median (quartile): 0.58 (0.17-1.27), 0.32 (0.17-0.61), 0.33 (0.13-0.62)] were significantly lower than that

关 键 词：砷中毒 煤 FOXP3 免疫因子 

分 类 号：R599.1[医药卫生—内科学]