辅助性T细胞-17与调节性T细胞在燃煤污染型砷中毒患者外周血中的比例变化及意义  

Changes and significance of the ratio of T helper 17 and regulatory T cell in peripheral blood of patients with coal-burning-borne arsenic poisoning

作　　者：夏仕青 方晓琳 朱凯[1] 董令[1] 邹忠兰 刘永莲[1] 王大朋[1] 张爱华[1] Xia Shiqing;Fang Xiaolin;Zhu Kai;Dong Ling;Zou Zhonglan;Liu Yonglian;Wang Dapeng;Zhang Aihua(Key Laboratory for Environmental Pollution Monitoring and Disease Control,Ministry of Education,Department of Toxicology,Guizhou Medical University,Guiyang 550025,China)

机构地区：[1]贵州医科大学环境污染与疾病监控教育部重点实验室卫生毒理学教研室,贵阳550025

出　　处：《中华地方病学杂志》2019年第2期101-106,共6页Chinese Journal of Endemiology

基　　金：国家自然科学基金(81430077、81730089).

摘　　要：目的观察燃煤污染型砷中毒患者外周血中辅助性T细胞-17(Thelper17,Th17)、调节性T细胞(regulatory T cell,Treg)占淋巴细胞的百分比及Th17/Treg细胞比值变化,探讨Th17、Treg细胞平衡在砷致免疫损伤机制中的作用。方法采用病例-对照研究方法,以贵州省黔西南州雨樟燃煤污染型砷中毒病区为调查点,选择149例砷中毒患者为病例组,年龄为(50.69±6.14)岁,其中男性94例,女性55例;并按照《地方性砷中毒诊断》(WS/T211-2015)标准将其划分为轻度砷中毒组(39例)、中度砷中毒组(54例)和重度砷中毒组(56例);以距离病区约12km的非砷暴露村的41例居民为对照组,年龄为(45.76±7.88)岁,其中男性12例,女性29例。采集调查对象发样及外周血,采用电感耦合等离子体质谱仪(ICP-MS)检测发砷含量,流式细胞术检测外周血中Th17、Treg细胞占淋巴细胞的百分比,并分析各组Th17/Treg细胞比值变化情况。结果对照组,轻、中、重度砷中毒组发砷含量[中位数(四分位数):0.12(0.08~0.18)、0.20(0.16~0.33)、0.25(0.18~0.41)、0.28(0.21~0.50)μg/g]组间比较差异有统计学意义(H=52.22,P<0.01),且各砷中毒组均高于对照组(P均<0.05)。中、重度砷中毒组人群外周血中Th17细胞占淋巴细胞的百分比[(0.42±0.21)%、(0.41±0.23)%]高于对照组[(0.29±0.16)%,P均<0.05];轻、中、重度砷中毒组人群外周血中Treg细胞占淋巴细胞的百分比[(0.37±0.18)%、(0.31±0.19)%、(0.27±0.18)%]低于对照组[(0.71±0.20)%,P均<0.05],且重度砷中毒组低于轻度砷中毒组(P<0.05);轻、中、重度砷中毒组Th17/Treg细胞比值(1.17±0.63、1.56±0.69、1.83±0.85)高于对照组(0.43±0.22,P均<0.05),且重度砷中毒组高于轻度砷中毒组(P<0.05)。相关性分析结果显示,发砷含量与外周血中Th17细胞占淋巴细胞的百分比和Th17/Treg细胞比值均呈正相关关系(r=0.323、0.608,P均<0.05);与Treg细胞占淋巴细胞的百分比呈负相关关系(r=-0.486,P<0.05)。�Objective To observe the change of T helper 17 (Th17), regulatory T cell (Treg) as a percentage of lymphocytes and the Th17/Treg ratio in peripheral blood of patients with coal-burning-borne arsenic poisoning, and to explore the role of Th17 cells and Treg cells balance in arsenic-induced immune injury. Methods A case-control study was conducted to investigate 149 cases of arsenic poisoning in Yuzhang arsenic poisoning area in the southwestern Guizhou Province, and the age was (50.69 ± 6.14) years old, including 94 males and 55 females. The diagnosis was based on the "Diagnosis of Endemic Arsenicosis" (WS/T 211-2015), and the cases were divided into mild arsenic poisoning group (39 cases), moderate arsenic poisoning group (54 cases) and severe arsenic poisoning group (56 cases);forty-one residents of non-arsenic exposed villages about 12 km away from the diseased area were collected as control group, the age was (45.76 ± 7.88) years old, including 12 males and 29 females. Hair samples and peripheral blood were collected from the subjects. The content of hair arsenic was detected by inductively coupled plasma mass spectrometry (ICP-MS). The percentages of Th17 cells and Treg cells in peripheral blood lymphocytes were detected by flow cytometry, and changes in the ratio of Th17/Treg in each group were analyzed. Results The hair arsenic contents in control, mild, moderate, and severe arsenic poisoning groups [median (quartile)] were 0.12 (0.08-0.18), 0.20 (0.16-0.33), 0.25 (0.18-0.41), 0.28 (0.21-0.50) μg/g, and the differences were statistically significant between groups (H=52.22, P < 0.01), and the hair arsenic content in each arsenic poisoning group was higher than that of the control group (P < 0.05). The percentages of Th17 cells in peripheral blood lymphocytes of moderate and severe arsenic poisoning groups [(0.42 ± 0.21)%, (0.41 ± 0.23)%] were higher than that of the control group [(0.29 ± 0.16)%, P < 0.05]. The percentages of Treg cells in peripheral blood lymphocytes of mild, moderate and severe arse

关 键 词：砷中毒 煤 辅助性T细胞-17 调节性T细胞 

分 类 号：R599.1[医药卫生—内科学]