直接稀释进样-电感耦合等离子体质谱法测定血清碘试剂条件的研究  

A study on the reagent condition for measuring iodine in serum by direct dilution sampling-inductively coupled plasma mass spectrometry

作　　者：黄淑英[1] 张亚平[1] 李呐[1] Huang Shuying;Zhang Yaping;Li Na(Clinical Laboratory,Xiamen Center for Disease Control and Prevention,Xiamen 361021,China)

机构地区：[1]福建省厦门市疾病预防控制中心检验科,361021

出　　处：《中华地方病学杂志》2019年第2期155-160,共6页Chinese Journal of Endemiology

基　　金：福建省医学创新项目课题(2012-CXB-38).

摘　　要：目的筛选适合于直接稀释进样-电感耦合等离子体质谱法(ICP-MS)测定血清中碘的试剂体系。方法采用6组不同稀释剂稀释血清样和标准系列溶液后,应用ICP-MS直接测定碘含量;比较内标元素铼或碲对测定结果的影响,与现行测定血清碘的砷铈催化分光光度标准方法(WS/T572-2017,简称标准方法)测定结果进行比较。检验所选试剂条件建立的方法在0~300μg/L碘浓度范围的标准曲线线性关系、测定检出限、精密度、准确度。结果经筛选建立的直接稀释进样-ICP-MS测定血清碘方法:以2.0g/L抗坏血酸-1.0g/L氯化铵-0.10%乙醇胺-1.0%乙醇溶液为稀释剂,标准系列溶液及血清样按样品∶稀释剂=1∶19稀释后,以铼为内标元素上机测定。0~300μg/L碘浓度范围标准曲线线性相关系数为0.9999,血清中碘的检出限为1.2μg/L(取样量为0.20ml)。精密度:对5份不同血清样测定的批内变异系数范围为0.2%~1.2%(n=6);批间变异系数范围为0.4%~1.9%(n=3)。准确度:对8份不同血清样的加标回收率范围为93.9%~103.8%,总平均回收率为97.9%;与标准方法同时测定35份血清样的结果比较差异无统计学意义(t=0.178,P>0.05)。结论选铼为内标元素测碘的准确度优于碲,所选试剂体系测定血清碘无需基体匹配,标准曲线线性好、检测灵敏度高、精密度与准确度优良、抗干扰能力强,操作简单,分析速度快,适宜推广应用。Objective To screen a suitable reagent system for measuring iodine in serum by direct dilution sampling-inductively coupled plasma mass spectrometry (ICP-MS). Methods Experiments were on sample dilution method for standard solutions and serum samples with six different diluents. After dilution, the concentration of iodine in serum was determined by ICP-MS method. The rhenium (Re) and tellurium (Te) was used as internal standard element, respectively. The effects of the two internal standard elements on determination results were compared. And the results were compared with the results determined by the current serum iodine arsenic cerium catalytic spectrophotometry standard method (WS/T 572-2017), hereinafter referred to as the standard method. The methodological evaluation of this new method was done through standard curve linearity, sample detection limit, precision and accuracy in determining serum iodine in the range of 0-300 μg/L. Results The direct dilution sampling-ICP-MS method screened for measuring iodine in serum was preparing 1 L of 2.0 g/L ascorbic acid, 1.0 g/L ammonium chloride, 0.10% ethanolamine, and 1.0% ethanol as diluent;the standard solutions and the serum samples were all diluted in a ratio of 1∶19 (sample ∶ diluent) before testing with Re as internal standard element. The linear range of the calibration curve was 0-300 μg/L and the linear correlative coefficient was 0.999 9. The detection limit for serum iodine was 1.2 μg/L (0.20 ml of serum was tested). Precision: the range of the intra assay coefficient of variation (CV) was 0.2%-1.2% (n=6) and the range of the inter assay CV was 0.4%-1.9% (n=3) when measuring 5 serum samples. Accuracy: The average recovery was 97.9% with a range of 93.9%-103.8% when measuring 8 serum samples. No significant difference was found between the results of the 35 serum samples determined by the standard method and the new method (t=0.178, P > 0.05). Conclusions Re performs better than Te in determination of iodine as an internal standard element. The re

关 键 词：碘 血清 电感耦合等离子体质谱法 稀释剂 

分 类 号：R446.1[医药卫生—诊断学]