一种溶酶体靶向双光子亚硝酰氢荧光探针的合成及细胞成像研究  

Lysosome-Targeted Dual-Photon Nitroxyl Fluorescent Probe: Synthesis and Application in Living Cell Imaging

作　　者：王晓芬 魏超[1] 李雪艳 郑雪阳 耿晓维 张平竹[1] 李小六[1] Wang Xiaofen;Wei Chao;Li Xueyan;Zheng Xueyang;Geng Xiaowei;Zhang Pingzhu;Li Xiaoliu(Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002)

机构地区：[1]河北大学化学与环境科学学院药物化学与分子诊断教育部重点实验室河北省化学生物学重点实验室

出　　处：《有机化学》2019年第2期469-474,共6页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(Nos.21572044,21778013);河北省自然科学基金(Nos.B2016201031,2018201234);河北省高等学校科学技术研究(No.QN2017015);保定市科学研究与发展计划(No.16zg031)资助项目~~

摘　　要：亚硝酰氢(HNO)是一氧化氮单电子还原并质子化的产物,具有重要的生物学意义.以4-(2-氨基乙基)-吗啉作为溶酶体靶向基团,1,8-萘二甲酰亚胺作为双光子荧光团,三苯基膦作为HNO识别基团,构建了一个能够特异性定位于溶酶体的打开型双光子荧光探针Lyso-HNO.研究结果表明,该探针响应迅速,对HNO表现出良好的选择性,较高的灵敏性,检测极限可达202 nmol·L^-1.该探针可对HeLa细胞溶酶体外源HNO进行双光子荧光成像研究.Nitroxyl (HNO), which is the one-electron reduced and further protonated form of nitric oxide, plays important biological functions. A lysosome-targeted dual-photon HNO fluorescent probe (Lyso-HNO), which contains 4-(2-amino-ethyl)morpholine as lysosomal-targetable groups, 1,8-naphthalimide as two-photon fluorophore and triphenylphosphine as HNO reaction site, was synthesized and characterized. The recognition behaviors of Lyso-HNO to HNO were investigated. The results showed that Lyso-HNO exhibited good selectivity and sensitivity to HNO with fast response,. and the detection limit of Lyso-HNO to HNO was estimated to be 202 nmol·L^-1. The probe can be applied to bioimaging exogenous lysosomal HNO by two-photon fluorescence confocal microscopy.

关 键 词：溶酶体 亚硝酰氢 双光子 细胞成像 

分 类 号：O657.3[理学—分析化学] Q2-33[理学—化学]