黄芩甙体外诱导大鼠骨髓基质细胞成为神经细胞  

Rat bone marrow stromal cells differentiate into neurons induced by baicalin in vitro

作　　者：贾延劼[1] 杨于嘉[1] 宋元宗[2] 

机构地区：[1]中南大学湘雅医院儿科,湖南长沙410008 [2]暨南大学医学院第一附属医院围产医学中心

出　　处：《中国病理生理杂志》2002年第3期247-249,T002,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .39870 2 51 );中国博士后科学基金资助项目

摘　　要：目的 :探讨黄芩甙体外诱导成年大鼠骨髓基质细胞 (MSCs)分化为神经细胞的条件。方法 :采用黄芩甙诱导 6h后 ,继续维持诱导 6d。免疫细胞化学染色评价神经细胞特异性烯醇化酶 (NSE)、神经微丝 (NF)、胶质纤维酸性蛋白 (GFAP)和波形蛋白 (vimentin)的表达率 ;Hoechst332 58染色评价细胞存活率。结果 :诱导 6d后MSCs形态改变 ,胞体成锥形 ,突起交织成网。免疫细胞化学染色NSE、NF、GFAP和vimentin表达率分别为 70 5 %± 1 1 6 % ,68 3 %± 1 3 4% ,<1 % ,<1 % ,细胞存活率为 88 4%± 5 0 %。结论 :黄芩甙可以诱导成年大鼠MSCs在体外分化成为神经细胞 。AIM: To study the protocol and condition that induce bone marrow stromal cells (MSCs) to differentiate into neuron in vitro by baicalin, a kind of flavonoid isolated from an important medicinal plant Scutellariae Radix . METHODS: MSCs from adult rats were induced by baicalin in serum-free medium for 6 h, and postinduced for 6 d. The morphological changes of MSCs were evaluated by light microscope. The positive percentages of neuron-specific enolase (NSE), 200-kilodalton neurofilament (NF), glial fibrillary acidic protein (GFAP) and vimentin expression were measured by immunocytochemistry with ABC staining. Hoechst 33258 staining was used to measure the cell viability by fluorescence microscope. RESULTS: After induction for 6 d, MSCs displayed neuronal morphologies, such as pyramidal cell bodies and processes formed extensive networks. The positive percentages of NSE, NF, GFAP and vimentin protein expression were 70.5%±11.6%, 68.3%±13.4% ,<1%, <1%, respectively. The cell viability was 88.4%±5.0%. CONCLUSION: Baicalin may induce adult rat MSCs to differentiate into neuron in vitro . It is a powerful approach to further cell transplantation and gene therapy in nervous system.

关 键 词：黄芩 骨髓 基质细胞 神经元 大鼠 黄芩甙 体外诱导 神经系统细胞移植 基因治疗 

分 类 号：R285.5[医药卫生—中药学]