系统性红斑狼疮患者外周血单个核细胞分泌白细胞介素-10干扰素-γ水平的研究  

Level and significance of Interleukin-10 and Interferon-γ secreted by peripheral blood mononuclear cells in patients with systemic lupus erythematosus

作　　者：王美美[1] 许桦[1] 许晋[1] 

机构地区：[1]东南大学附属中大医院风湿免疫科,南京210009

出　　处：《中华风湿病学杂志》2002年第1期28-30,共3页Chinese Journal of Rheumatology

摘　　要：目的　通过检测系统性红斑狼疮 (SLE)患者外周血单个核细胞 (PBMC)分泌细胞因子白细胞介素 10 (IL 10 )、干扰素 γ (IFN γ)水平 ,探讨T淋巴细胞亚群与SLE发病关系。方法　采用酶联免疫吸附法 (ELISA)测定 30例SLE患者和 10名健康志愿者PBMC培养上清IL 10和IFN γ水平。结果　①SLE活动组PBMC自发分泌IL 10水平明显升高 ,与非活动组、对照组比较差异有显著性 (P <0 0 0 1) ;非活动组明显高于对照组 (P <0 0 1)。②PBMC分泌IFN γ水平三组 (SLE活动组、非活动组和对照组 )比较差异无显著性 (P >0 0 5 )。结论　IL 10、IFN γ作为区分CD4T细胞亚群的指标之一 ,间接反映了Th1、Th2细胞活化状态。本文研究结果显示 ,SLE患者PBMC分泌IL 10水平明显增高 ,且增高程度与病情活动性相关 ,IFN γ分泌水平无明显变化。提出Th2型细胞因子介导的免疫应答在SLE的发病机制中占主导地位 ,即“Th2优势应答”。我们认为PBMC分泌IL 10水平对SLE诊断和病情活动性监测均有一定临床意义。寻找有效方法调节SLE患者体内IL 10水平 ,从而调节Th1/Th2平衡 ,将为治疗SLE开辟一条新途径。Objective To investigate interleukin 10 (IL 10,Th2 type cytokines) and interferon gamma (IFN γ,Th1 type cytokines) secreting capacity by peripheral blood mononuclear cells (PBMCs) in 30 patients with systemic lupus erythematousus (SLE) and 10 healthy individuals,and try to understand the role of Th1/Th2 balance in the pathogenesis of SLE.Method Sandwich ELISA was used to measure IL 10 and IFN γ level in the supernatant of culture from PBMCs.Results The level of IL 10 produced spontaneously by PBMCs in active stage was significantly higher than in static phase and healthy person ( P <0 001).There was significantly difference between active and inactive patients ( P <0 01).The IFN γ level was not statistically different between SLE patients and controls ( P >0 05).Conclusion These data suggest that the Th2 type cytokine (IL 10) was increased in patients with SLE,and an imbalance towards Th2 predominance may play a significant role in the pathogenesis of SLE.Regulating IL 10 level may provide a new approach for the treatment of SLE.

关 键 词：系统性红斑狼疮 白细胞介素10 干扰素Ⅱ型 PBMC IFNΓ 

分 类 号：R593.241[医药卫生—内科学]