道地药材建泽泻的RAPD研究  

Studies on DNA fingerprint of genuine Chinese herbs Alisma orientalis by RAPD

作　　者：胡珊梅[1] 周涵韬[2] 张启国[1] 阮元彬[2] 

机构地区：[1]厦门市药品检验所,福建厦门361012 [2]厦门大学生命科学学院,福建厦门361000

出　　处：《中草药》2002年第2期161-162,共2页Chinese Traditional and Herbal Drugs

基　　金：厦门市卫生局医学科学技术进步基金科研资助项目

摘　　要：目的　为了探讨道地药材与非道地药材之间遗传变异的大小 ,并建立道地药材的品质鉴定方法。方法　运用了随机扩增多态 DNA( RAPD)技术 ,对福建、江西、四川产的泽泻科植物泽泻 Alisma orientalis进行了研究。结果　同种异地药材所形成的不同的居群 ,具有不同的遗传特征。结论　 RAPD技术可作为鉴别道地药材的参考方法。Object To research genovariations between genuine Chinese herbs and non genuine ones and to develop a valuable tool used in identification of the Chinese herb.Methods RAPD technique was applied in studies on the samples of Alisma orientalis (Sam.) Juzep. from different area in Fujian, Sichuan and Jiangxi provinces.Results The DNA fingerprints of genuine and non genuine Chinese herbs were compared and it was suggested that the herbal populations growing in different area in above three provinces had different genus characteristics.Conclusion RAPD technique is a valuable tool for identification of genuine Chinese herbs.

关 键 词：泽泻 道地药材 RAPD 居群 遗传变异 研究 中药 

分 类 号：R282.71[医药卫生—中药学]