阿霉素纳米粒的制备及体外逆转人膀胱癌细胞多药耐药的实验研究  

Preparation of doxorubicin-loaded nanoparticles and reversion of MRP mediated MDR of human bladder tumor cell line in vitro

作　　者：张炎[1] 鲁功成[1] 张润清[1] 陈晓春[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院泌尿外科,武汉430022

出　　处：《临床泌尿外科杂志》2002年第3期122-123,共2页Journal of Clinical Urology

摘　　要：目的 :合成阿霉素聚氰基丙烯酸异丁酯纳米粒 (DOX PIBCA NP)并检测其相关参数 ,观察其对多药耐受相关蛋白 (MRP)介导的人膀胱癌细胞多药耐药 (MDR)的逆转。方法 :以乳液聚合法合成阿霉素纳米粒 ,透射电镜计算粒径 ,分光光度法计算包封率 ,MTT法观察MDR的逆转。结果 :阿霉素纳米粒平均粒径 98.5± 3.6 7nm ,包封率 95 % ,其对耐药、敏感株的细胞毒作用差异无显著性意义 (P >0 .0 5 ) ,耐药株对阿霉素纳米粒较对阿霉素敏感 4 .9倍 (P <0 .0 5 )。结论 :阿霉素纳米粒可有效逆转MRP介导的MDR。Purpose:To prepare doxorubicin nanoparticles and study the characteristics of the nanoparticles and the reversion of MRP mediated multidrug resistance of human bladder carcinoma cell lines in vitro.Method:The size of nanoparticles was determined by electron transmission microphotography,the percentage of doxorubicin associated with the nanoparticles was deternined by spectrophotometer,the cytotoxicity of DOX,DOX PIBCA NP and PIBCA NP were detenined by measuring the inhibition of cell growth using the MTT assay.Result:DOX PIBCA NP showed the same cytotoxic effect for sensitive and resistant cells,the MDR Lines were 4.9 times more sensitive to DOX PIBCA NP than to doxorubicin.Conclusion:DOX PIBCA NP was effective to overcome the multididrug resistance.

关 键 词：聚氰基丙烯酸异丁酯纳米粒 阿霉素 膀胱肿瘤 多药耐受相关蛋白 体外逆转 实验研究 

分 类 号：R737.14[医药卫生—肿瘤]