幼龄皱纹盘鲍消化道粘膜上皮的超微结构与组织化学  

Ultrastructure and histochemistry of alimentary canal mucosal epithelium of larval abalone Haliotis discus hannai

作　　者：卞建春 崔龙波[2] 周雪莹[3] 陆瑶华[3] 

机构地区：[1]扬州大学畜牧兽医学院,江苏扬州225009 [2]东北农业大学生命科学学院,黑龙江哈尔滨150030 [3]烟台大学生物化学系,山东烟台264005

出　　处：《中国水产科学》2002年第1期10-13,共4页Journal of Fishery Sciences of China

基　　金：国家自然科学基金资助项目 ( 39470 35 1)

摘　　要：以透射电镜与组织学方法研究 4 5日龄皱纹盘鲍 (Haliotisdiscushannai,壳长 2 .3 0～ 2 .4 0mm ,壳宽1.80～ 2 .10mm)的消化道。结果表明 :①食道分为前、中、后 3段 ;中段分为食物通道和食道侧囊 ,食道侧囊的分泌细胞含大量的囊泡和分泌颗粒 ,呈强的蛋白酶和非特异性酯酶活性 ;食道前段、食物通道及食道后段粘膜上皮由纤毛细胞和粘液细胞组成。②嗉囔粘膜上皮只有 1种细胞 ,细胞游离缘具有长达 4 .0 μm的密集微绒毛。③胃粘膜上皮由纤毛细胞和微绒毛细胞组成。④肠和直肠粘膜上皮由 5种细胞组成 ,即纤毛细胞、微绒毛细胞、分泌细胞、颗粒状腺细胞和粘液细胞 ,分泌细胞基部有少量的分泌颗粒。⑤存在于消化道各部位的微绒毛细胞游离缘均具密集的微绒毛 ,游离端质膜呈碱性磷酸酶活性 ;食道和肠的粘液细胞分泌粘多糖 ;食道、胃和肠的纤毛细胞游离缘具纤毛。The atimentary canal mucosal epithelium of 45 day old larval abalone Haliotis discus hannai (body length 2.30-2.40 mm and body width 1.80-2.10 mm) were observed under transmission electronic microscope and by histochemical method. The results show that:① The esophagus consists of three sections which are anterior section, middle section and rear section; the middle section consists of food canal and lateral pouch; the cells secreted from the lateral pouch contain large numbers of vesicles and secretory granules which show strongly positive activities of protease and non specific esterase; the mucosal epithelium of esophagus anterior section, food canal and esophagus rear section consists of ciliated cells and mucous cells.② There is only one kind of cell on the crop mucosol epithelium, and there are dense microvilli as long as 4.0 μm at the free end of the cell.③ The stomach mucosal epithelium consists of ciliated cells and microvilli.④ The intestine and intestinum rectum mucosal epithelium consist of five types of cells, i.e. ciliated cell, microvillus, secretory cell, granular gland cell and mucous cell. The secretory cells contain a few of secretory granules in the basal region.⑤ The microvilli cells in every section of alimentary canal have dense microvilli and the plasmalemma at the free ends shows ALK activity, and the mucous cells of food canal and intestine secrete mucopolysaccharide, also, in the alinentary canal, stomach and intestine, there exist some cilia at the free ends of the ciliated cells.

关 键 词：皱纹盘鲍 幼鲍 消化道 粘膜上皮 超微结构 组织化学 

分 类 号：S944.45[农业科学—水产养殖]