花鲈弧菌病病原菌(哈维氏弧菌)的分离与鉴定  

Isolation and identification of pathogen (Vibrio harveyi) from sea perch, Lateolabrax japonicus

作　　者：王保坤[1] 余俊红[1] 李筠[1] 纪伟尚[1] 徐怀恕[1] 

机构地区：[1]青岛海洋大学海洋生命学院,山东青岛266003

出　　处：《中国水产科学》2002年第1期52-55,共4页Journal of Fishery Sciences of China

基　　金：国家自然科学基金资助项目 ( 39870 5 81) ;国家重点基础研究发展规划"973"资助项目 (G19990 12 0 0 4)

摘　　要：1999年 4～ 5月 ,山东省青岛市胶南海区网箱养鱼场花鲈 (Lateolabraxjaponicus)幼鱼发生暴发性传染病 ,死亡率达 5 0 %。从具有明显症状的病鱼的病灶组织分离到 1株优势菌SF 1,经人工感染和从人工感染发病的花鲈再分离的SF 3菌株的再感染试验结果表明 ,所分离的菌株为此次花鲈烂尾病的致病菌。经形态、生理生化等 64项特征指标鉴定 ,SF 1和SF 3均为哈维氏弧菌 (Vibrioharveyi)。药敏试验结果表明 ,头孢噻肟、头孢三嗪、头孢孟多、呋喃妥因、氯霉素、氧哌嗪青霉素、磺胺类、复方磺胺、三甲氧苄氨嘧啶、多粘菌素E等Mass motilities of sea perch (Lateolabrax japonicus) occurred in Jiaonan fishery farms, Qingdao, Shandong Province from April to May 1999. The main syndromes of the diseased fish are rotting fins and rotting tails. One strain of bacteria SF 1 was isolated from the diseased sea perch, and another strain SF 3 was isolated from the diseased sea perch challenged by SF 1 by muscle injection. Most of the morphological characteristics of these two isolations are the same. They were identified as Vibrio harveyi by 64 test items physiological and biochemical characteristics. The sensitive test shows that among 33 chemotherapeutants, the pathogen are resistant to 21 and sensitive to chloramphenicol, ceftriaxone, cefotaxime, cefamandole, nitrofurantoin, trimethoprim,piperacillin,sulfonamides,sulfamethoxazole,and polymyxin E, etc.

关 键 词：花鲈 哈维氏弧菌 药敏试验 弧菌病 病原菌 分离 鉴定 海水养殖 鱼类 

分 类 号：S943.211[农业科学—水产养殖] S941.42[农业科学—水产科学]