火龙果茎段离体培养快速繁殖试验  

Rapid Propagation of Hylocereus undatus cv.vietnam by In Vitro Stem Sections

作　　者：周传明[1] 黄寿先[1] 熊英[1] 黄文 

机构地区：[1]广西大学林学院,南宁市邕武16号530001

出　　处：《广西科学》2002年第1期78-80,共3页Guangxi Sciences

摘　　要：选取火龙果当年春季抽出的未老熟茎段作外植体 ,以 MS为基本培养基 ,诱导腋芽生长及继代培养时附加 6 - BA和 NAA,6 - BA浓度为 1.0～ 12 .0 mg/L,NAA浓度为 0 .2～ 0 .8m g/L;诱导生根时附加 IBA,IBA浓度为 0 .5 mg/L、 1.0 mg/L、 1.5 mg/L。试验结果表明 :以 MS基本培养基附加 6 - BA2 .0 mg/L+NAA0 .2 mg/L 有利于诱导腋芽生长 ;附加 6 - BA12 .0 mg/L+NAA0 .1mg/L 有利诱导增殖和继代 ;附加 IBA1.5 mg/L 诱导生根最好 ,生根培养 7d后开始生根 ,培养 2 0 d生根率达 96 .7%。将火龙果生根苗移栽到菜园土和细砂基质 ,成活率均达 10 0 % ,以细砂为基质的移栽苗木生长较好 ,说明火龙果的组培苗移栽需要有良好的透水 。Tender stems of Hylocereus undatus cv vietnam are taken as explants MS medium(MS) is used and added with 6 BA of 1 0 to 12 0 mg/L and NAA of 0 2 to 0 8 mg/L to induce axillary buds'growth and subculture, and supplemented with IBA at the concentrations 0 5 mg/L,1 0 mg/L and 1 5 mg/L to induce rooting The experiment shows that MS+6 BA 2 0 mg/L+NAA 0 2 mg/L is suitable for growth of axillary buds, and MS+6 BA 12 0 mg/L+NAA 0 1 mg/L suitable for subculture and, MS+IBA1 5 mg/L suitable for induction of rooting The shoots root on 7th day after transplanted, and reach 96 7% of rooting rate on 20th day after transplanted All plantlets are survival when they are transplanted into the soil of vegetable garden and sand medium,but the plantlets grow better in the sand medium than in the soil

关 键 词：火龙果 茎段 组织培养 繁殖试验 离体培养 

分 类 号：S667.9[农业科学—果树学]