新风胶囊对佐剂性关节炎大鼠脾脏及胃黏膜细胞超微结构作用的实验研究  

Experimental study on the effect of Xinfeng capsule(新风胶囊)on the ultrastructure of spleenlymphocyte and gastric mucosa cell of the rat with adjuvantinduced arthritis

作　　者：刘健[1] 郑志坚[2] 韩明向[1] 雷黎[1] 胡闻[3] 

机构地区：[1]安徽中医学院第一附属医院,安徽合肥230031 [2]卫生部北京医院 [3]安徽省立医院

出　　处：《中国中西医结合急救杂志》2002年第2期76-78,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：卫生部优秀青年科研基金资助项目 (No.992 6)

摘　　要：目的 :观察新风胶囊对佐剂性关节炎大鼠的治疗作用及对脾脏淋巴细胞和胃黏膜细胞超微结构的影响。方法 :将 6 0只大鼠随机分为正常对照组、模型对照组、新风胶囊组和雷公藤组 ,每组 15只。制备完全佐剂并分别向除正常对照组以外其余动物的右后足跖皮内注射 0 .1ml致炎 ;电镜观察脾脏淋巴细胞及胃黏膜细胞超微结构变化 (包括线粒体肿胀、空泡变、嵴突病变等 ) ,计算各组线粒体病变率。结果 :与给药前相比 ,新风胶囊组给药后模型大鼠体重显著增加 (P<0 .0 5 ) ,而雷公藤组体重无显著变化 (P>0 .0 5 )。与模型组相比 ,新风胶囊组脾脏淋巴细胞及胃黏膜细胞线粒体病变率显著降低 (P均 <0 .0 5 ) ,而雷公藤组脾脏淋巴细胞线粒体病变率无显著改善 (P均 >0 .0 5 ) ,胃黏膜细胞线粒体病变率显著提高 (P均 <0 .0 5 )。与雷公藤组相比 ,新风胶囊组给药后脾脏淋巴细胞及胃黏膜细胞线粒体病变率均显著降低 (P均 <0 .0 5 )。结论 :新风胶囊能增加佐剂性关节炎大鼠体重 ,其改善模型大鼠脾脏及胃黏膜细胞的超微结构病变的作用优于雷公藤。新风胶囊的作用机制可能与恢复和维持脾脏正常结构 ,改善免疫调节功能和微循环 ,保护胃黏膜 ,进而改善模型大鼠的整体状况有关。Objective:To observe the effect of Xinfeng capsule (XFC,新风胶囊)on the ultrastructure of spleenlymphocyte and gastric mucosa cell of the rat with adjuvantinduced arthritis(AA).Methods:Sixty rats were randomly divided into normal control group,model control group,XFC group and tripterygium wilfordii polycoside tablet (TPT,雷公藤多甙片) group.Every group had 15 rats.Complete adjuvant was prepared and 0.1 ml of it was injected into rightposterior subcutaneous part of every animal's pediphalanx except normal control animals.Electron microscope was used to assess ultrastructural lesion of spleenlymphocyte and gastric mucosa cell (including chondriosome swelling,vacuolation,and ridge pathologic change).The chondriosome lesion rate of every group was measured respectively.Results:Compared with before treatment,the weight of rats in XFC group after treatment increased markedly ( P <0 05),and the weight of rats in TPT group had no marked change ( P >0 05).Compared with model control group,the chondriosome lesion rate of spleenlymphocyte and gastric mucosa cell in XFC group was lower (all P <0 05),but chondriosome lesion rate of spleenlymphocyte in TPT group was not marked variation(all P >0 05),and chondriosome lesion rate of gastric mucosa cell in TPT group was marked higher (all P <0 05).Compared with TPT group,chondriosome lesion rates of spleenlymphocyte and gastric mucosa cell in XFC group were marked lower (all P <0 05).Conclusions:XFC is superior to TPT in gaining weight and improving ultrastructural lesion of spleen and gastric mucosa cell.The mechanisms are related to the restoration and maintenance of normal structure of spleen,regulation of immune function,improvement of microcirculation and protection of gastric mucosa.

关 键 词：佐剂性关节炎 新风胶囊 脾脏 胃 超微结构 中医药疗法 

分 类 号：R285[医药卫生—中药学]