二苯胺法定量检测细胞凋亡  

The Apoptosis Quantitative Detection by Diphenylamine

作　　者：刘艳红[1] 刘先洲[1] 邓兆群[1] 王焰山 汤纪路[1] 白瑞珍[1] 

机构地区：[1]武汉大学医学院微生物学教研室,武汉430071 [2]湖北省咸宁卫校,咸宁437100

出　　处：《武汉大学学报（医学版）》2002年第1期63-65,共3页Medical Journal of Wuhan University

基　　金：湖北省卫生厅资助项目 (9740 2 )

摘　　要：目的 :建立二苯胺定量检测细胞凋亡的实验方法。方法 :以地塞米松体外作用 5h的小鼠胸腺细胞作为凋亡阳性细胞 ,应用二苯胺法测定细胞片段化DNA和总DNA的含量 ,二者的比值定义为细胞凋亡率。结果 :地塞米松 (1× 10 - 5mol L)处理 5h后 ,细胞凋亡率为 30 .2 4 %。此与流式细胞仪分析结果 (2 7.10 % )基本一致。结论 :建立了一种简单、经济。Objective: To establish the method of the apoptosis quantitative detection by diphenylamine. Methods:The apoptosis of separated mice thymocytes were induced by treating 5 hours of dexamethasone (1×10 -5 mol/L). The ratio of DNA fragments to the total of DNA detected by diphenylamine method was defined to the positive rate of apoptosis. Results:The apoptosis positive rate of mice thymocytes was 30.24% after treated 5 hours by dexamethasone ,which was close to the result of flow cytometry analysis(27.10%). Conclusion:We have built a simple, cheap and rapid method for quantitative detection of apoptosis.

关 键 词：细胞凋亡 DNA分析 二苯胺 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]