胆汁泡蛋白促成核作用及其主体活性成分的分析  

The study of pro-nucleating activity on 33.5 ×103 vesicular protein in bile

作　　者：马保金[1] 项建斌[1] 蔡端[1] 张延龄[1] 查锡良[2] 王丽影[2] 

机构地区：[1]复旦大学附属华山医院外科 [2]复旦大学基础医学院生化教研室

出　　处：《中华外科杂志》2002年第4期298-300,共3页Chinese Journal of Surgery

基　　金：国家自然科学基金项目 (3 95 70 695 );卫生部科学研究项目 (98 2 12 4)

摘　　要：目的　分析 33 5× 10 3 泡蛋白促成核活性以及多肽、糖链改变对其活性的影响。　方法　制备人工模拟胆汁 ,在偏光显微镜下观察结晶成核生长过程 ,计算成核活性 ;酶法水解蛋白多肽与糖链结构 ,通过结晶生长试验检测 33 5× 10 3 泡蛋白促结晶生长作用以及糖链水解、多肽降解产物的活性改变。　结果　 33 5× 10 3 泡蛋白具有强促成核活性 ,成核活性强达 0 310 ,在不同模拟胆汁体系中 ,成核活性基本相同 ;其结晶生长试验活性指数It、Ig、Ic分别为 0 5 7、1 5 2及 1 6 3(P <0 0 5 )。糖苷酶切、肽链酶解后其成核活性均几乎丧失。　结论　 33 5× 10 3 泡蛋白可能参与成石过程 ,其中多肽、糖链调节其成核活性。Objective To evaluate the pro nucleating activity in 33 5 ×10 3 vesicular protein. Methods The model biles were established according Kibe and Zhu. The pro nucleating activitiy of 33 5 ×10 3 vesicular protein were examined by polarized light microscopy. The protein and its enzymatic deglycosylation and proteolysis fractions nucleation promoting activity were detected by cholesterol crystal growth assay. Results 33 5×10 3 vesicular protein displayed apparent potency of pro nucleation with activity of 0 310, and derived crystal growth curve indices It, Ig, Ic were presented as 0 57,1 52,1 63 respectively, but after treated by N glycanase enzyme and pronase, no promoting activity were found. Conclusion The 33 5×10 3 vesicular protein may be involved in the nucleation process of gallstone formation, which is regulated by its peptide and sugar chain

关 键 词：胆结石 胆汁 成核效应蛋白 泡蛋白 糖链 

分 类 号：R341[医药卫生—基础医学]