多西紫杉醇体外诱导骨肉瘤细胞系pl-1的凋亡  

Apoptosis of osteosarcoma cell line pl-1 indnced by docitaxol in vitro

作　　者：彭磊[1] 王臻[1] 王庆良[1] 曹云新[1] 朱德生[1] 张志培[1] 吴银松[1] 施新猷[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032

出　　处：《中国临床药理学与治疗学》2002年第1期1-4,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金资助 (№ 3 9770 749)

摘　　要：目的 :研究多西紫杉醇对骨肉瘤细胞生长抑制及诱导凋亡作用。方法 :应用细胞计数、形态学观察、蛋白含量测定、流式细胞术和电镜等方法对多西紫杉醇诱导的骨肉瘤进行检测和观察。结果 :多西杉醇在浓度为 4mg·L- 1时 ,对骨肉瘤细胞有明显的生长抑制作用 ,并且存在着时间依赖关系和一定范围内的剂量依赖关系。多西紫杉醇可将骨肉瘤细胞阻断于G1期 ,并诱导凋亡 ,其凋亡细胞具有典型凋亡细胞特征。用流式细胞仪测定细胞周期 ,可见明显的凋亡峰。电镜观察染色质沿皱缩的核膜下凝聚 ,细胞表面出现凋亡小体 ,基质呈絮状致密化 ,紫杉醇作用短时间去除后再培养比持续作用更易促使细胞凋亡。结论 :多西紫杉醇对骨肉瘤细胞的细胞毒作用是诱导其凋亡的结果 ,这种诱导凋亡的能力是紫杉醇疗效的基础 ,在对骨肉瘤的治疗中 。AIM: To study the growth inhibition and apoptosis inducted by docitaxol in osteosarcomatous cells. METHODS:Docitaxol induced osteosarcomatous cells were detected and observed with cell counting, morphologic observation, flow cytometry (FCM), and electron microscope etc. RESULTS: Docitaxol could inhibit the growth of osteosarcomatous cells effectively at the concentration of 4 mg·L -1 in a time dependent manner and further dose dependent manner in a certain range. Osteosarcomatous cells were blocked during G1/M phage and induced to apoptosis. Cells undergoing apoptosis exhibited the following typical features; an apparent apoptoic peak during dection of cell cycle with FCM, chromatin's cling to periphery of the shrunken nuclear, apoptitic body, and floccule condensed matrix with electron microscope. Further, cells recultureed after being treated with docitaxol shortly were more apt to undergo apoptosis than cells treated with docitaxol continually. CONCLUSION: Because of the ability to induce apopotisis, docitaxl shows a great potential in the treatment of osteosarcoma.

关 键 词：多西紫杉醇 骨肉瘤 细胞凋亡 抗癌药 中药 植物药 

分 类 号：R286.91[医药卫生—中药学] R738.1[医药卫生—中医学]