阿米替林对谷氨酸损伤中枢神经细胞的保护作用  

Protective effects of amitriptyline on glutamate-induced neurotoxicity in cerebral neuronal cultures

作　　者：徐庆荣[1] 

机构地区：[1]山东省滨州医学院,滨州256603

出　　处：《中国临床药理学与治疗学》2002年第1期24-26,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :研究阿米替林 (Ami)对谷氨酸损伤培养大鼠皮层神经细胞的保护作用。方法 :分离培养15～ 18d胎龄的大鼠神经细胞 ,加入谷氨酸 (Glu) ,造成神经细胞的损伤 ,测定乳酸脱氢酶、一氧化氮、丙二醛及超氧化物歧化酶的含量。观察谷氨酸对神经细胞的损伤作用及阿米替林的保护作用。结果 :加入谷氨酸后 ,神经细胞出现明显损伤性变化 ,死亡率升高 ,培养上清液中乳酸脱氢酶 (LDH)、一氧化氮(NO)含量增加 ,细胞匀浆中丙二醛 (MDA )生成增加 ,超氧化物歧化酶 (SOD)含量明显减少。Ami 10 - 8～ 10 - 6 mol·L- 1能不同程度地减轻上述损伤性变化。结论 :Ami对谷氨酸所致的神经细胞损伤具有保护作用 。AIM: To study the protective effects of amitriptyline (Ami) on glutamate induced neurotoxicity in cultured rat cerebral cortical neurons. METHODS: Cortical neurons of fetal rat were cultured in vitro. The protective effects of Ami on glutamate induced neurotoxicity were observed. RESULTS: Exposure of rat fetus cerebral cells to Glu medium developed a neurotoxicity, expressed in the increase of LDH, NO and MDA, and the decrease of activity of SOD,as well as the development of morphological injury. Ami (10 -6 ,10 -7 , 10 -8 mol·L -1 ) significantly protected neurons against above demage. CONCLUSION: Ami can prevent rat cerebral neurons injury from the toxicity of Glu.

关 键 词：阿米替林 谷氨酸 大脑皮层神经细胞 细胞培养 动物实验 

分 类 号：R971[医药卫生—药品]