未成熟树突状细胞诱导异基因嵌合体的形成  

Immature dendritic cells alone can induce mixed allogeneic chimerism

作　　者：于平[1] 熊思东[1] 何球藻[1] 储以微[1] 郑秀娟[1] 

机构地区：[1]复旦医学院免疫学系上海基因免疫与疫苗研究中心,上海200032

出　　处：《中国免疫学杂志》2002年第4期223-227,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金重点项目资助(No.39830340)

摘　　要：目的：证实供体来源的未成熟树突状细胞（ｉｍＤＣｓ）主动免疫受体小鼠后可诱导形成异基因嵌合体。方法：应用供体（Ｃ５７ＢＬ／６）来源的骨髓细胞在体外培养出ｉｍＤＣｓ，灭活后静脉输注受体小鼠（ＢＡＬＢ／ｃ），并于输注后不同时间输注供体来源的骨髓细胞，检测异基因嵌合体的形成。同时比较ｉｍＤＣｓ一次免疫与多次免疫对诱导嵌合体形成的影响。结果：在ｉｍＤＣｓ免疫后３ｄ输注骨髓细胞可诱导异基因嵌合体的形成，并持续１００ｄ以上；在免疫后５ｄ注射骨髓细胞只能形成短暂的（＜２１ｄ）、低水平（＜１％）的嵌合状态；７ｄ后则完全不能形成嵌合。应用一次和多次ｉｍＤＣｓ免疫均能诱导形成异基因嵌合体，且维持时间均大于１００ ｄ，但其嵌合程度有显著性统计学差异（Ｐ＜０．０５）。结论：应用供体来源的ｉｍＤＣｓ主动免疫受体可诱导形成异基因嵌合体。Objective:To explore the possibility of immature dendritic cells (imDCs) in the induction of mixed allogeneic chimerism in recipients without irradiation or cytoreduction treatment. Methods: Bone marrow cells (BMCs) derived from donor (C57BU6) were used to generate imDCs. imDCs were injected to the recipients (BALB/c)i v. Fresh-made bone marrow cells from donor were then transfused i.v to the recipients treated with imDCs at different time points. The different levels of chimerism induced by single or multiple injections of imDCs were studied. Results: imnDCs can induce mixed allogeneic chimerism, which was observed at different time points after donor-derived BMCS injection. However, only those injected the BMCs at the third day after the immunization with imDCs could lead to significantly longer mixed alogeneic chimerism( > 100 d) than those injected BMCs at the fifth day (<21 d), and the recipients who received BMCs at the seventh day could not induce chimerism at all. Although all injections induced a long- tenn chimerism, the levels of chimedsm were significantly different in the recipients after the single,twice and three times injection of imDCs. COnclusion: hnniature dendritic cells alone can induce the generation of mixed allogeneic chimerism in allogeneic recipients without irradiation and other cytoreduction host treatment.

关 键 词：未成熟树突状细胞 异基因嵌合体 移植免疫 免疫耐受 

分 类 号：R392.12[医药卫生—免疫学] R392.4[医药卫生—基础医学]