基质金属蛋白酶及其抑制剂和转化生长因子-β_1在中耳胆脂瘤的表达  

Expression of transforming growth factor-β_1 matrix metalloproteinase-1 and its inhibitor in human middle ear cholesteatoma

作　　者：杨向茹[1] 李西秦[2] 马敏[2] 张莉萍[2] 张芩娜[1] 王建明[1] 王斌全[1] 

机构地区：[1]山西医科大学第一医院耳鼻咽喉,太原030001 [2]山西医科大学第二医院耳鼻咽喉科

出　　处：《中华耳鼻咽喉科杂志》2002年第2期121-123,共3页Chinese Journal of Otorhinolaryngology

基　　金：山西省科委资助项目 (972 181)

摘　　要：目的　探讨基质金属蛋白酶 1(matrixmetalloproteinase 1,MMP 1)及其抑制剂金属蛋白酶组织抑制剂 1(tissueinhibitorofmetalloproteinase 1,TIMP 1)和影响二者作用的转化生长因子 β1(transforminggrowthfactor 1,TGF β1)在中耳胆脂瘤中的表达。方法　采用免疫组化技术检测MMP 1、TIMP 1及TGF β1在中耳胆脂瘤 (41例 )和外耳道骨部正常皮肤 (15例 )中的表达。结果　TGF β1、MMP 1及TIMP 1在中耳胆脂瘤中的阳性表达率分别为 95 12 %、85 37%、9 76 % ,与外耳道正常皮肤相比 ,TGF β1和MMP 1的表达均差异有显著性 ,而TIMP 1的表达差异无显著性。TGF β1与MMP 1表达间存在正相关 ,与TIMP 1表达间无相关性。结论　MMP 1与TIMP 1表达间的失衡 。Objective To investigate the expression of matrix metalloproteinase 1 (MMP 1), tissue inhibitor of metalloproteinase 1 (TIMP 1) and transforming growth factor β 1 (TGF β 1) in human middle ear cholesteatoma Methods Immunohistochemical staining was used to detect the expression of TGF β 1, MMP 1 and TIMP 1 in the cholesteatoma (41 cases) and the skin of external auditory meatus (15 cases) Results The percent of positive expression of TGF β 1, MMP 1 and TIMP 1 in cholesteatoma was 95 12%, 85 37% and 9 76%, respectively There was significant difference of the expression of MMP 1 and TGF β 1 between cholesteatoma and external auditory meatus skin ( P <0 05) But there was no statistic significance for TIMP 1 ( P >0 05) There was correlation between the expression of TGF β 1 and MMP 1, but no correlation between TGF β 1 and TIMP 1 Conclusion The imbalance between the expression of MMP 1 and TIMP 1 in cholesteatoma may cause directly proteolytic activity involved in pathologic osteolytic mechanisms

关 键 词：中耳胆脂瘤 金属蛋白酶类 转化生长因子Β 骨质吸收 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]