p-NiMe_4TAA化学修饰电极的制备及其在测定胶囊中褪黑素中的应用  

Poly - NiMe_4TAA Modified Electrode for Melatonin Detection

作　　者：高梦南[1] 潘振声[1] 徐芳[1] 王琳[1] 金利通[1] 林丽[2] 

机构地区：[1]华东师范大学化学系,上海200062 [2]温州医学院化学教研室,温州325027

出　　处：《化学传感器》2002年第1期15-21,共7页Chemical Sensors

摘　　要：本文通过电化学聚合法制备了聚四甲基二苯并四氮杂轮烯镍(p-NiMe_4TAA)修饰电极,该电极对褪黑素有良好的催化氧化效果。用循环伏安法(CV)研究了修饰电极的催化氢化行为,将该电极用示差脉冲伏安法(DPV)测定褪黑素,在5.0×10^(-5)mol/L～5.0×10^(-7)mol/L浓度范围内与氧化峰电流呈良好线性关系,检测限达1.0×10^(-7)mol/L(S/N=3)。将该法用于检测美乐宁胶囊中褪黑素的含量,得到了令人满意的结果。In this paper, the electropolymerization of NiMe4TAA( Nickel (Ⅱ) tetramethyldibenzo [b, i] tetraaza[14] annulene) on glassy carbon electrodes has been performed in non -aqueous solution. The poly - NiMe4TAA modified electrode(p - NiMe4TAA ME) showed efficient electro-catalytic properties to melatonin oxidation and its response was evaluated with respect to polymerization cycle numbers and pH of the electrolyte. A differential pulse voltammetry (DPV) method was developed for the quantitative determination of melatonin in 0. 1 mol· L-1 phosphate buffer solution (PBS, pH 7. 4) on the p - NiMe4TAA ME. The DPV peak current showed a linear relationship with the melatonin concentration in the range of 5. 0×10-5mol·L-1- 5. 0×10-7 mol· L-1 with a correlation coefficient of 0. 9993 and a detection limit of 1.0×10-7mol·L-1 (S/N = 3). The method was applied to assay melatonin in capsules with satisfactory results.

关 键 词：褪黑素 聚四甲基二苯并四氮杂轮烯镍 p-NiMe4TAA 修饰电极 胶囊 测定 

分 类 号：O657.1[理学—分析化学]