人细胞生长相关5个新基因的染色体定位及其基因结构  

Chromosome Localization and Genomic Organization of Five Novel Human Genes Related to Cell Growth Control

作　　者：何祥火[1] 覃文新[1] 王俊茹[1] 胡建[1] 朱洪新[1] 万大方[1] 顾健人[1] 

机构地区：[1]上海市肿瘤研究所癌基因及相关基因国家重点实验室,上海200032

出　　处：《遗传》2002年第2期111-116,共6页Hereditas（Beijing)

基　　金：国家重点基础研究发展规划项目 (G19980 5 12 0 9)

摘　　要：基因的染色体定位对我们研究基因相互关系、基因的组织与进化及理解基因与疾病关系具有重要的意义。本文采用RH -PCR方法及生物信息学方法对PP3898、PP115 8、PP75 3、SP2 6 0、HC5 6等 5条人细胞生长相关新基因进行染色体定位 ,并分析了其基因结构。PP3898及PP115 8定位于 19p13 3,PP75 3及SP2 6 0定位于 1q2 1 1,HC5 6定位于 17p13 3。PP3898含有 19个外显子和 18个内含子 ,可读框为 2 5 6 5bp ;PP115 8含有 7个外显子和 6个内含子 ,可读框为 12 18bp ;SP2 6 0含有 10个外显子和 9个内含子 ,可读框为 6 90bp ;HC5 6为单外显子 ,可读框为3141bp。另外 。Five novel human genes related to cell growth control were newly isolated and identified by high-throughput functional screening.In this paper,the chromosomal localization of these five genes is reported.Radiation hybrid mapping and in silico mapping,and their genomic organization were analyzed respectively.PP3898 and PP1158 were assigned to chromosome 19p13.3,SP260 and PP753 to chromosome 1q21.1,and HC56 to chromosome 17p13.3.PP3898 contains nineteen exons and eighteen introns,PP1158 seven exons and six introns,SP260 ten exons and nine introns,and HC56 only one exon.The implications of chromosomal localization are discussed.

关 键 词：放射杂交 染色体定位 基因结构 人类基因组 

分 类 号：Q987[生物学—遗传学]