应用抑制性消减杂交技术克隆大鼠肝再生过程中特异表达基因  

Cloning Differentially Expressed Genes by Suppression Subtractive Hybridization in Rat Liver Regeneration

作　　者：李玉昌[1] 徐存拴 张云汉[2] 

机构地区：[1]河南师范大学生命科学学院,新乡453002 [2]河南省肿瘤病理重点实验室,郑州450052

出　　处：《遗传》2002年第2期152-154,共3页Hereditas（Beijing)

基　　金：国家自然科学基金 ( 39970 36 2 )资助项目

摘　　要：应用抑制性消减杂交技术成功地构建了高消减效率的正向消减cDNA文库 ,从随机挑取的 5 0个克隆中有31个均检出了 6 0～ 40 0bp插入片段 ,对这些插入cDNA片段进行测序后经Genbank同源性检索 ,表明其中 7个片段为未知新序列。大鼠肝切除后肝再生cDNA正向消减文库的建立为进一步大批量筛选、克隆肝再生特异性表达的未知新基因奠定了基础 。The cDNA from rat regenerating liver tissue was used as the tester and that from normal liver was used as the driver.A highly efficient subtractive cDNA library was constructed by suppression subtractive hybridization(SSH).After screening,31 clones from 50 clones which were derived from the cDNA library were inserted by 60～400bp cDNA fragments.24 cDNA fragments corresponded to known genes and 7 cDNA fragments were unknown sequences(GenBank accession number:BG447490～447496).

关 键 词：抑制性消减杂交 消减文库 部分肝切除 肝再生 同源性检索 大鼠 细胞增殖 再分化 

分 类 号：Q953[生物学—动物学]