转录后基因沉默的机制及其应用  

The Mechanism and Application of Posttranscriptional Gene Silencing

作　　者：刘杨[1] 蒋彦[1] 乔代蓉[1] 曹毅[1] 

机构地区：[1]四川大学草原生物工程国家实验室,成都610064

出　　处：《生物工程学报》2002年第2期140-143,共4页Chinese Journal of Biotechnology

基　　金：国家转基因植物及产业化专项 (No .J0 0 A 0 0 8 0 9);国家自然科学基金 (No .39780 0 18)~~

摘　　要：确定导致转录后基因沉默的原因 ,探索在转基因植物研究中避免基因沉默的对策。方法是将转基因沉默分为转录水平的沉默和转录后水平的沉默 ,分别对RNA阈值模型、异位配对和异常RNA模型、双连RNA模型等几种导致转录后基因沉默模型的分析。结果确定了转基因沉默抑制现象和转录后基因沉默的形成机制 ,以及转录后基因沉默理论和实践意义。The purpose of this review is to confirm the reason resulted the gene silence and explore the countermeasure avoiding the gene silence in transgene plant.The method is to divide the gene silencing into transcriptional gene silencing(TGS) and post transcriptional gene silencing(PTGS).Several models resulted PTGS were analyzed by RNA threshold model,ectopic pairing and aberrant RNA model and ds RNA model.The results showed that it was important to decide the phenomena of restraining transgene silencing and the mechanism of PTGS .The strategies of identification of gene function and prevention of virus were presented by RNAi and gene silencing respectively,etc.

关 键 词：转录后基因沉默 基因沉默 aRNA RDRP 异位配对 沉默抑制因子 RNAI 机制 

分 类 号：Q753[生物学—分子生物学] Q756