肾综合征出血热原代地鼠肾细胞灭活疫苗纯化方法的研究  

A Method for Purifying Inactivated Hemorrhagic Fever with Renal Syndrome(HFRS) Vaccine Prepared with Golden Hamster Kidney Cell (GHKC)

作　　者：韩亮[1] 王琪[1] 冉姝[1] 赵丽红[1] 惠连[1] 王晓宏[1] 回良杰[1] 赵健[1] 马军[1] 郝富勇[1] 王伟[1] 姜惠贤[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》2002年第2期87-90,共4页Chinese Journal of Biologicals

摘　　要：目的 建立简便、适合大规模肾综合征出血热灭活疫苗的纯化方法。方法 采用超滤浓缩、醋酸锌沉淀和柱层析（亲和层析、离子交换层析和凝胶过滤层析）等方法，对出血热原代地鼠肾细胞灭活疫苗进行提纯对比试验。结果 用超滤浓缩和Sepharose 4FF凝胶柱层析或用醋酸锌沉淀和凝胶柱层析，纯化的疫苗均可达到质量标准，而用 cellufine sulfate gel亲和层析和离子交换层析均不理想。结论 建立了经济、适合大规模肾综合征出血热灭活疫苗的纯化方法。[Abstract] Objective To establish a simple method suitable for the large - scale purification of inactivated hemorrhagic fever with renal syndrome (HFRS) vaccine. Methods The inactivated HFRS vaccine prepared with primary golden hamster kidney cells (GHKC) was purified by various methods such as ultrafiltra-tion, precipitation with zinc acetate, affinity, ion - exchange and gel filtration chromatography, and the results of them were compared. Results The purities of vaccines purified ultrafiltration plus Sepharose 4FF column chromatography or precipitation widi zinc acetate plus Sepharose 4FF column chromatography met Chinese Requirements for Biologies. However, those purified by ceEufine sulfate gel affinity or ion- exchange chromatography were unsatisfied. Conclusion An economic and simple method suitable for the large - scale purification of inactivated HFRS vaccine is established.

关 键 词：肾综合征出血热病毒 柱层析 疫苗 纯化方法 原代细胞 地鼠 肾细胞 

分 类 号：R373.32[医药卫生—病原生物学] R512.8[医药卫生—基础医学]