响应面分析法用于微生物培养基浓度的优化  

Application of respones surface method for optimal concentration of culture medium for microorganisms

作　　者：胡永红[1] 沈树宝[1] 欧阳平凯[1] 

机构地区：[1]南京化工大学,制药与生命科学学院南京210009

出　　处：《工业微生物》2002年第1期9-12,共4页Industrial Microbiology

摘　　要：首次将响应面分析法 (RSM )用于延胡索酸酶产生菌产氨短杆菌MA 2和黄色短杆菌MA 3培养基成份的优选 ,并且取得了良好的结果。经优化后产氨短杆菌MA 2酶活达 2 7.88×10 3 μmol/g·h ,黄色短杆菌MA 3酶活达 32 .2 6× 10 3 μmol/g·h ,较国内外文献报道的其它产氨短杆菌、黄色短杆菌单位菌体延胡索酸酶活力有显著提高。The response surface method (RSM) was firstly applied to determine and optimize the components of the culture medium for the two selected strains. The enzyme activities of fumarase under the optimum conditions for B. ammoniagenes MA 2 and B. flavum MA 3 were 27.88ⅹ10 3 μmol/g.h and 32.26ⅹ10 3 μmol/g.h respectively, which were higher than those reported in the literature.

关 键 词：响应面分析法 培养基 优化 浓度 微生物 

分 类 号：Q93-335[生物学—微生物学]