用壳聚糖替代Sepharose 4B固定相关配体纯化酶的研究  

Purification of enzyme through affinity chromatograpy using chintosan as ligand carrier

作　　者：周毅彬[1] 张培德[1] 

机构地区：[1]复旦大学生物化学系,上海200433

出　　处：《工业微生物》2002年第1期19-22,共4页Industrial Microbiology

摘　　要：采用壳聚糖固定酶作用的特定底物 (菌体细胞 ) ,并用戊二醛交联制备成酶的亲和吸附剂。采用该吸附剂纯化溶葡球菌酶的研究表明 ,经一步纯化可提高纯度 4倍 ,酶活性回收大于70 %。SDS PAGE的电泳结果显示 ,产品基本上达到了标准酶的纯度。同时表明该吸附剂没有非特异性吸附。由载体壳聚糖替代Sepharose 4B制备的吸附剂具有简单、快速、较高收得率和操作安全等优点 ,适用于特定酶或基因工程产品的分离纯化。A affinity chromatography technique using chitosan as ligand carrier to immobilize Staphylococcus aureus to purify lysostaphin was presented. which was bound to chitsan by glutaraldehyde. The results of affinity chromatography showed that the recovery of enzyme activity reached 70% and the purity examined by SDS PAGE reached the standard of lysostaphin without any non specific binding. This affinity chromatography column could be used repeatedly and the purification efficiency was not decreased,which had the advandages of safety, simplicity and quickness.

关 键 词：壳聚糖 固定化 金黄色葡萄球菌 溶葡球菌酶 溶菌酶 配体 

分 类 号：O629[理学—有机化学]