PKC在慢性低氧大鼠肺动脉重构中的作用  

AN EXPERIMENTAL STUDY ON THE REGULATION OF PULMONARY ARTERIAL REMODELING BY PROTEIN KINASE C IN CHRONIC HYPOXIC RATS

作　　者：周浩[1] 陈少贤[1] 王良兴[1] 陈彦凡[1] 谢于鹏[1] 王群姬[1] 范小芳[1] 

机构地区：[1]温州医学院附属一医呼吸内科,肺心病研究室,浙江温州325000

出　　处：《中国应用生理学杂志》2002年第1期38-42,共5页Chinese Journal of Applied Physiology

摘　　要：目的 :探讨蛋白激酶C(PKC)在慢性低氧大鼠肺动脉重构中的作用。方法 :采用透射电镜、放射活性测定法、免疫组化、图像分析等方法综合进行评价。结果 :①肺动脉平均压 (mPAP)、右心室重量比 (RV LV +S)显著高于对照组 (P <0 .0 1) ;②光镜下肺细小动脉管壁面积 管总面积 (WA TA)、肺细小动脉中膜平滑肌细胞核密度 (SMC)显著高于对照组 (P <0 .0 1) ;电镜显示肺动脉中膜平滑肌细胞增生 ,胶原纤维较对照组明显为多 ;③肺组织PKC总活性(PKCt)、胞膜PKC活性 (PKCm)、胞浆PKC活性 (PKCc)及PKCm PKCt的百分比显著高于对照组 (P <0 .0 1) ;④免疫组化显示肺细小动脉 (直径约 10 0～ 2 0 0 μm)PKC含量、Ⅰ型胶原含量显著高于对照组 (P <0 .0 1) ,Ⅲ型胶原组间无明显差异 (P >0 .0 5 ) ;⑤肺组织PKCt、PKCm、PKCm PKCt和肺动脉管壁PKC的表达与肺细小动脉中膜平滑肌细胞核密度 (SMC)、肺动脉管壁Ⅰ型胶原的表达均呈正相关。结论 :PKC参与慢性低氧肺动脉平滑肌细胞增殖、管壁胶原表达的调控 。Aim: To investigate the effect of protein kinase C regulating pulmonary arterial remodeling in chronic hypoxic rats.Methods: Electron microscope, radioactity, immunohistochemistry and image analysor were used . Results: ① Mean pulmonary arterial pressure (mPAP)and weight ratio of RV to LV+S were significantly higher than that of control group (P<0.01).② WA/TA and SMC were significantly higher than that of control group (P<0.01). Electron microscopy showed the proliferation of smooth muscle cells and the diposition of collgenous fiber in pulmonary arterioles induced by hypoxia.③ The total,cytosolic,particulate fraction PKC activity and the ratio of particulate fraction to total PKC activity were significantly higher than that of control group (P<0.01).④ Expression of PKC,collagen Ⅰ were significantly higher than that of control group (P<0.01), the difference of collagen Ⅲ was not significant between two groups(P>0.05).⑤There were good correlation between the total,particulate fraction PKC activity, the ratio of particulate fraction to total PKC activity,expression of PKC and SMC,collagen Ⅰin pulmonary arterioles.Conclusion: The PKC regulates the proliferation of pulmonary artery smooth muscle cells and expression of pulmonary arterial collagen in chronic hypoxic rats,which may play an important role in the pathogenesis of pulmonary hypertension and structural remodeling of pulmonary arteries.

关 键 词：蛋白激酶C 低氧 肺性高血压 平滑肌细胞 胶原 PKC 肺动脉重构 

分 类 号：R543.202[医药卫生—心血管疾病]