皮质酮对高钾诱导PC12细胞内钙升高的非基因组调节作用  

NONGENOMIC ACTION OF CORTICOSTERONE TO CALCIUM RISE INDUCED BY HIGH-K^+ IN PC12 CELLS

作　　者：娄淑杰[1,2] 黄秀英[3] 韩建忠 陈菲[1] 陈宜张[1] 路长林[1] 

机构地区：[1]第二军医大学神经生物学教研室,神经科学研究所,上海200433 [2]中科院生理所神经生物学开放研究实验室,北京100081 [3]中国科学院发育生物学研究所

出　　处：《中国应用生理学杂志》2002年第1期47-50,共4页Chinese Journal of Applied Physiology

基　　金：中科院生理所神经科学开放实验室 ( 0 10 111);国家自然科学基金资助项目 ( 139930 0 80 )

摘　　要：目的 :分析皮质酮快速抑制高钾诱导PC12细胞内钙升高的机制。方法 :使用荧光影像系统 ,实时检测细胞内钙变化。结果 :①在预处理PC12细胞 5min后 ,皮质酮可呈量 -效关系抑制高钾诱导的PC12细胞内钙升高。②牛血清白蛋白耦联的皮质酮 (B BSA)可模拟皮质酮快速抑制高钾诱导PC12细胞内钙升高的作用 ,并呈现量 -效关系。③糖皮质激素的细胞内受体拮抗剂RU38486对皮质酮快速抑制效应无明显拮抗作用。④蛋白合成抑制剂放线菌酮预处理细胞 3h后 ,皮质酮对高钾诱导PC12内钙升高仍有较强抑制作用。结论 :①皮质酮的作用点位于细胞膜上。②皮质酮的快速作用不直接依赖细胞内蛋白合成和不依赖细胞内糖皮质激素受体。Aim: To analyse the mechanism of corticosterone on the elevation of cytosolic free calcium (i) induced by high-K + in pheochromocytoma PC12 cells, Methods: The i was real-time checked by fluorescenc image system.Results: ①When the cells were preincubated at 37℃ for 5 min in the presence of various concentration corticosterone and stimulated with 55 mmol/L KCl , an inhibitory effect of corticosterone on Δi was observed in a concentration-dependent manner. ② When PC12 cells were preincubated with various concentration of B-BSA at 37℃ for 5 min and stimulated with 55 mmol/L KCl, an inhibitory effect of B-BSA on Δi was observed, which is also concentration-dependent manner. ③ The inhibitory effect of corticosterone and B-BSA could not be antagonized by RU 38486 at 10 -4 mol/L. ④cycloheximide could not block the inhibitory effect of corticosterone after pretreating cells at 10 -5mol/L at 37℃ for 3 hours. Conclusion: It is obvious that the locus of corticosterone action is on the plasmic membrane. The inhibitory effect of corticosterone is independent of protein synthesis and intracelullar glucocorticoid receptor. The effect of corticosterone on i is nongenomic action in PC12 cells.

关 键 词：皮质酮 高钾 细胞内钙离子浓度 非基因组作用 PC12细胞 非基因组机制 调节作用 

分 类 号：Q577[生物学—生物化学]