大鼠卵巢不同功能阶段时c-fos表达的动态变化  

EXPRESSION OF c-fos IN VARIOUS FUNCTIONAL STATE OF RAT OVARIES

作　　者：郑月慧[1] 吴凌峰[1] 钟志胜[1] 方廉[1] 吴绮明[1] 

机构地区：[1]江西医学院生理教研室,江西南昌330006

出　　处：《中国应用生理学杂志》2002年第1期88-92,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目 ( 396 6 0 0 2 7)

摘　　要：目的和方法 :本文用免疫组织化学方法检测了成年大鼠卵巢于不同的功能阶段和未成年大鼠外源性激素诱导的卵巢于不同的功能阶段c fos表达的变化以及与血清E2 和P含量的相关关系。结果 :在成年大鼠动情前期、动情期卵巢的间质腺和基质中及动情间期和妊娠期卵巢的黄体细胞和基质中有c fos表达 ,c fos蛋白阳性信号的表达范围和表达强度在动情前期卵巢较大 ,动情间期和动情期卵巢较低 ,妊娠期卵巢最大 ,这种变化与血清E2 和P含量的变化呈明显的正相关关系。未成年大鼠 ,用DES使卵泡发育至窦前期时 ,在卵巢中未检测到有c fos蛋白的表达 ,用PMSG使卵泡发育至排卵前期时在卵巢的间质腺和基质中有c fos表达 ,用PMSG和hCG后 4天形成的早期黄体化卵巢c fos在黄体细胞和基质中的表达明显升高 ,而于用hCG后 9天形成的晚期黄体化卵巢c fos表达明显下降 ,这种变化也与血清E2 和P含量的变化呈明显的正相关关系。结论 :c fos与卵泡发育、排卵。Aim and Methods: The method of labeled stretavidin biotin was used to study the expression of c-fos in various functional state of rat ovaries and its relationship with the levels of serum estrodial and progesterone. Results: ①In the mature rat,c-fos expression was found higher in interstitial gland and stroma of proestrous ovaries and lower in estrous ovaries ,and was found higher in luteal cells and stroma of pregrnant ovaries and lower in diestrous ovaries.There was a positive correlation between the area and optical density of c-fos expression and the levels of serum E 2 and P.② In the immature rats,c-fos expression was not found in the ovaries containing preantral follicles from DES-treated rats,and was found both in interstital gland and stroma of the ovaries containing preovulatory follocles from PMSG-treated rats ,and the expression was higher in day 4 luteinized ovaries and lower in day 9 luteinized ovaries from PMSG with hCG treated rats. There also was a positive correlation between the area and optical density of c-fos exprssion and the levels of serum E 2 and P. Conclusion: The results suggested that c-fos existed in rat ovaries and might play an important role in follicles development,ovulation,luteum formation and regression.

关 键 词：大鼠 C-FOS 卵巢 雌二醇 孕酮 激素分泌 功能 表达 

分 类 号：Q575.12[生物学—生物化学] Q492.5