HCV5′NCR转基因细胞HepG2.9706的特性分析  

The Biological Characteristics of HCV 5′NCR Transgenic Cell HepG2.9706

作　　者：王小红[1] 王升启[1] 管伟[1] 毛秉智[1] 

机构地区：[1]军事医学科学院放射医学科学院,北京100850

出　　处：《病毒学报》2002年第1期74-76,共3页Chinese Journal of Virology

基　　金：国家"8 6 3"基金资助 (编号 :10 2 -0 8-0 4-0 1) ;军队9 5重点课题 (编号 :96Z0 0 7)基金资助

摘　　要：In order to investigate the biological characteristics of the transgenic cellular model(HepG2.9706),the transgene copy numbers in HepG2.9706 were tested by complex probe fluorescent quantitative PCR and Southern hybridization.Its luciferase activity was assayed by luciferase assay system.The results showed that HepG2.9706,after consecutive passages for two years,keeps excellent luciferase activities and its transgene copy number is around 140 copies.This indicates that HepG2.9706 contains a high ratio of transgene integration and is very stable.In order to investigate the biological characteristics of the transgenic cellular model(HepG2.9706),the transgene copy numbers in HepG2.9706 were tested by complex probe fluorescent quantitative PCR and Southern hybridization.Its luciferase activity was assayed by luciferase assay system.The results showed that HepG2.9706,after consecutive passages for two years,keeps excellent luciferase activities and its transgene copy number is around 140 copies.This indicates that HepG2.9706 contains a high ratio of transgene integration and is very stable.

关 键 词：HepG2.9706细胞株 荧光定量PCR SOUTHERN杂交 拷贝数 荧光素 丙型肝炎病毒 转染 

分 类 号：R373.21[医药卫生—病原生物学]