新疆地区盐湖的中度嗜盐菌16S rDNA全序列及DNA同源性分析  

Analysis of 16S rDNA Sequences and DNA-DNA Hybridization of Moderately Halophilic Bacteria from Xinjiang Region

作　　者：曾静[1] 窦岳坦[1] 王磊[1] 杨苏声[1] 

机构地区：[1]中国农业大学生物学院微生物学系,北京100094

出　　处：《微生物学报》2002年第2期133-137,共5页Acta Microbiologica Sinica

摘　　要：通过数值分类和 1 6SrDNAPCR RFLP分析 ,对分离自新疆地区的中度嗜盐革兰氏阴性菌进行研究 ,发现了一个新类群。在此基础上 ,进行了中心株AI 3的 1 6SrDNA全序列分析 ,并与中度嗜盐菌已知种和相关种进行比较 ,得到系统发育树状图。在此树状图中 ,大多数参比菌株聚在一起 ,其 1 6SrDNA全序列的同源性在 96 %以上 ,而AI 3与参比菌株的 1 6SrDNA全序列相比 ,其相似性低于 75 %。但是 ,AI 3与Alcanivoraxborkumensis[1] 的 1 6SrDNA全序列的相似性为 96 % ,与Halobacilluslitoralis的 1 6SrDNA全序列的相似性为 99% ,三者构成一个独立的发育分支。这说明在系统发育上 ,AI 3与参比菌株属于不同的分支 ,是一个新的类群。在新类群内 ,菌株之间的DNA同源性大于 70 % ,而中心株AI 3与标准菌株伸长盐单胞菌(Halomonaselongata)的DNA同源性为 44% 。Based on the previous studies on numerical taxonomy and 16S rDNA PCR-RFLP analysis, the moderately halophilic bacteria isolated from Xinjiang Region constitued a new cluster, and the phylogenetic tree was constructed by comparing with the 16S rDNA sequences of the other moderately halophilic bacteria species. In the phylogenetic tree, most of the reference strains were clustered in a group, and the similarity values of 16S rDNA sequence were above 96%. However, AI-3, Alcanivorax borkumensis and Halobacillus litoralis were clusterd in another group, and the similarity value of 16S rDNA sequences between AI-3 and Alcanivorax borkumensis was 96%, and that of 16S rDNA sequences between AI-3 and Halobacillus litoralis was 99%. The results indicated that AI-3 was different from the reference strains in phylogeny. The values of DNA homology in the new cluster were more than 70%, but the value between AI-3 and Halomonas elongata was less than 50%. Thus, the strain AI-3 possibly represent a new moderately halophilic bacteria species.

关 键 词：新疆地区 16S rDNA全序列 DNA-DNA杂交 系统发育 盐湖 嗜盐菌 DNA同源性 

分 类 号：Q93-331[生物学—微生物学]