进化上保守的小鼠F-LANa基因的计算机克隆(简报)  

IN SILICO CLONING OF EVOLUTIONARILY CONSERVED MOUSE F-LANa

作　　者：应浩[1] 徐永华[1] 

机构地区：[1]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031

出　　处：《实验生物学报》2002年第1期59-61,共3页Acta Biologiae Experimentalis Sinica

基　　金：中国科学院人类基因组研究项目;国家重大基础研究特别基金(G1999053905)

摘　　要：EST(expressed sequence tag,表达序列标签)是对cDNA克隆进行测序时得到的序列,一般位于该克隆的一端,序列为单次测序所得到的。现在EST已成为一个非常大的数据库,并被广泛运用于基因家族新成员的寻找,也可作为人染色体上的标志来寻找多态性位点,还可以用来比较某基因在不同组织和病理状态中的表达模式。在EST数据库中,Differentially expressed genes between normal liver and hepatocellular carcinomas were investigated using differential display. In previous study, human F-LANa was identified as a differentially expressed gene, up-regulated in hepatocellular carcinoma. In this study, we developed an in silico cloning approach to rapidly and accurately characterize the mouse ortholog of the human F-LANa. Mouse F-LANa encodes a 239 aa protein exhibiting 97. 9 % similarity to the human ortholog gene. Homology analysis was carried out in various species and showed that F-LANa was evolutionary conserved from yeast to human. Based on the alignment results, phylogenetic tree was established here.

关 键 词：小鼠 F-LANa 计算机克隆 进化保守 系统树 基因克隆 

分 类 号：S188[农业科学—农业基础科学]