小麦细胞核仁中rRNA基因转录的超微定位(简报)  

ULTRASTRUCTURAL LOCALIZATION OF TRANSCRIPTION SITES OF rRNA GENES IN THE NUCLEOLUS OF WHEAT

作　　者：赫杰[1] 陶伟[2] 郝水[3] 

机构地区：[1]哈尔滨工业大学生命科学与工程系,哈尔滨150001 [2]北京大学生命科学学院,北京100871 [3]东北师范大学遗传与细胞研究所,长春130024

出　　处：《实验生物学报》2002年第1期71-75,共5页Acta Biologiae Experimentalis Sinica

基　　金：国家重点基础研究发展规划(973计划)资助项目(项目编号J1999053902)

摘　　要：真核细胞核仁中rRNA基因转录位点是长期以来未能解决的问题。以小麦细胞为研究材料,应用常规电子显微镜技术,观察了小麦细胞核仁纤维中心(Fibrillar centers,FC)内染色质的超微结构;并通过DNA抗体阐明了核仁中DNA位于纤维中心、致密纤维组分(Dense fibrillar component,DFC)以及两者的过渡区域;应用RNA聚合酶I相关转录因子UBF(Upstream binding factor)抗体所做的分析显示,小麦细胞核仁中UBF位于FC与DFC的过渡区域以及DFC中,在FC中没有UBF的存在;进一步借助于RNA/DNA杂合体抗体选择性地直接标记核仁中rRNA基因的转录位点,结果表明了小麦细胞核仁rRNA基因的转录位点是在FC与DFC的过渡区域及DFC中。There were no definite conclusions about transcription sites of rRNA genes in nucleoli of eukary-otes for a long time. We used the wheat as specimen and observed ultrastructure of chromatin in FC of nucleoli by conventional transmission electron microscope. And we employed anti-DNA antibodies to analyze the distribution of DNA in nucleoli, and found that DNA located in FC, DFC and the transitional region between them. Using anti-UBF antibodies to study localization of RNA polymerase I transcription factor UBF in nucleoli, we observed that UBF located in DFC and the transitional region between DFC and FC in wheat, but not in FC. Furthermore, with anti-RNA/DNA hybrid antibodies, we directly and selectively labeled the transcription sites of rRNA genes of nucleoli and revealed that it located in DFC and the transitional region between DFC and FC in wheat.

关 键 词：小麦 核仁 rRNA基因转录 转录因子UBF RNA/DNA杂合体 转录位点 超微定位 

分 类 号：S512.1[农业科学—作物学]