水稻双链RNA结合蛋白同源基因OsRBP的克隆及其表达的分析  

Isolation and Expressional Analysis of cDNA Encoding a dsRNA Binding Protein Homologue OsRBP of Rice

作　　者：唐向荣[1] 吴昊[1] 贾明[2] 余旭红[1] 何玉科[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,上海200032 [2]上海浦东新区农业技术推广中心,上海201201

出　　处：《植物生理与分子生物学学报》2002年第1期41-45,共5页Journal Of Plant Physiology and Molecular Biology

摘　　要：在基因数据库中发现两个水稻EST片段与大白菜BcpLH基因的双链RNA结合结构域 (dsRBD)有同源的区域 ,根据同源片段的位置特征设计引物 ,用RT-RCR扩增粳稻 (Oryzasativasubsp .japonica)愈伤组织的cDNA ,得到大小为 1.8kb的DNA片段。该cDNA片段含完整的编码区 ,有两个典型的dsRBD ,分别与BcpLH的dsRBD在氨基酸水平上同源性为 75 %左右 ,故将其命名为OsRBP。RT -PCR表达分析显示该基因在未成熟的种子和愈伤组织中表达 ,在根、茎、叶、穗、成熟种子及胚芽鞘中没有表达信号 ,由此推测该基因的表达可能与种子和胚的早期发育相关。该研究首次从水稻中分离到双链RNA结合蛋白基因 ,并初步研究了其表达方式 。Two EST fragments in GenBank were found to be homologous to double-stranded RNA binding domains of BcpLH gene of Chinese cabbage.The primers were designed according to the position of homologous regiogs and RT-PCR was performed using cDNA as the template of callus tissue of rice (Oryza sativa subsp.japonica ZH11).A 1.8-kb diagnostic DNA fragment spanning the complete coding sequence(Fig.1)of OsRBP was generated.It contains two typical dsRBD domains sharing 75% identity in amino acid sequence(Fig.3) and therefore was designated as OsRBP.It is showed by expression analysis of RT-PCR that the expression of was seen in immatued seeds and callus derived from immatured seeds and not found in roots,stems,leaves,spikes and matured seeds(Fig.4).The first repost on dsRNA binding protein gene and its expression pattern makes it possible to study regulatory function of dsRNA binding protein to development of rice major organs.

关 键 词：水稻 双链RNA结合蛋白 胚 RT-PCR OsRBP 基因克隆 基因表达 

分 类 号：S511.01[农业科学—作物学]