利用RAPD标记评价小豆种质遗传多样性  

Study on genetic diversity of adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi)germplasm based on RAPD markers

作　　者：王述民[1] 胡英考[2] 胡家蓬[1] 

机构地区：[1]中国农业科学院作物品种资源研究所,北京100081 [2]北京首都师范大学生物系

出　　处：《植物遗传资源科学》2002年第1期14-19,共6页

摘　　要：本研究利用10个 RAPD 引物对180份小豆种质的基因组 DNA 进行扩增,共扩增出44条带,其中35条具有多态性,比例为79.5%,平均每个引物扩增出3.5条多态性带;平均遗传距离为0.274,变异幅度为0.05～0.60,平均遗传多样性指数为0.692;基于 RAPD 标记,把180份小豆种质聚类划分为4个组群,该组群的划分与小豆的生态地域性似乎不存在明显的相关性。The analysis of the 180 adzuki accessions with 10 informative RAPD primers generated 44 scorable bands,35 (79.5 %) of which were polymorphic.One RAPD primer detected a mean of 3.5 polymorphic bands.The mean genetic distance among accessions was 0.274 with a range of 0.05 to 0.60 while the mean genetic diversity index was 0.692.Four groups were clustered based on the RAPD data by using hierarchical cluster analysis.However,no apparent correlation with putative geographic origin were detected.

关 键 词：小豆 RAPD标记 遗传多样性 聚类分析 种质资源 

分 类 号：S521[农业科学—作物学]