管藻目绿藻刺松藻(Codium fragile)PSI色素蛋白复合物结构及多肽组成  

STRUCTURE AND PEPTIDE COMPOSITION OF PSI PIGMENT-PROTEIN COMPLEXES OF SIPHOUNOUS GREEN ALGA, Codium fragile

作　　者：陈敏[1] 李爱芬[1] 周百成[2] 

机构地区：[1]烟台大学生物化学系,264005 [2]中国科学院海洋研究所,青岛266071

出　　处：《海洋科学》2002年第4期40-43,共4页Marine Sciences

摘　　要：采用温和的聚丙烯酰胺凝胶电泳 (PAGE)法 ,从管藻目绿藻刺松藻中分离到不同解离程度的光系统I(PSI)复合物 ,以及核心复合物CCI、捕光复合物LHCI和LHCI的亚组分 ,并就其多肽组成及相互关系做了研究。纯化的CCI复合物只含有66和56kDa多肽 ,荧光发射主峰在714～715nm ;LHCI的77K荧光峰在683～684nm ,没有高等植物730nm特征荧光 ,含有PSI复合物中的全部4种24.7～27.5kDa多肽。将中心复合物CPI再电泳 ,得到了只含有24.5和27.2kDa多肽的LHCI亚组分。从而证实刺松藻LHCI也可分裂为含有光合色素的两个部分 ,它们与CCI结合的紧密程度不同。By mild PAGE method, PSI complexes in varying decomposition degrees, core complex CCI, light harvesting complex LHCI and a LHCI subunit were resolved from a siphonous green alga, Codium fragile. The peptide composition and arrangement in these complexes were also investigated. Purified CCI complex contained only 66 and 56 kDa peptides, and its main fluorescence emission band at 77K was at 714～715 nm. LHCI contained all four peptides of 24.7～27.5 kDa presented in PSI complexes, and had a single fluorescence peak around 683～684 nm other than those at 730 nm in higher plants. When CPI was subjected to further PAGE, the LHCI subunit containing two 24.5 and 27.2 kDa peptides was isolated. It demonstrated that LHCI of C.fragile could be fractionated into two subunits containing photosynthetic pigments. The two subunits attached with CCI to a different extent.

关 键 词：PSI LHCI CCI 低温荧光 多肽排布 刺松藻 色素蛋白复合 管藻目绿藻 

分 类 号：Q949.218.1[生物学—植物学]