养殖牙鲆鳗弧菌疫苗的研究  

VACCINATION AGAINST Vibrio Anguillarum ON CULTURED FLOUNDER, Paralichthys olivaceus

作　　者：莫照兰[1] 徐永立[1] 张培军[1] 

机构地区：[1]中国科学院海洋所实验海洋生物学开放实验室,青岛266071

出　　处：《海洋科学》2002年第4期63-66,共4页Marine Sciences

基　　金：中国科学院海洋研究所调查研究报告第4211号;国家重点基础研究专项资助项目G1999012003号

摘　　要：以牙鲆病原性鳗弧菌M3为抗原 ,制备了细胞(CV)、细胞 胞外产物(CEV)、脂多糖(LPS)三种疫苗 ,通过浸泡和注射两种途径对牙鲆进行两次免疫 ,检测疫苗的免疫保护效果。免疫后4周可检测到免疫组牙鲆具有明显的凝集抗体效价 ,其中注射组效价显著高于浸泡组 ,而对照组变化不明显。与对照组相比 ,免疫组牙鲆获得良好的免疫保护力 ,其中注射免疫组高于浸泡组。在注射免疫途径中 ,以CEV的免疫保护效果最好 ;在浸泡免疫途径中 ,以CEV和LPS的免疫保护效果最好。结果表明鳗弧菌M3的细胞The efficacy of three vaccine formulations, a whole cell bacterin (CV), a whole cell with extrocellular products vaccine (CEV) and a lipopolysacchride vaccine (LPS), which are against Vibrio anguillarum M3 was evaluated by the route of immersion and injection in flounder, Paralichthys olivaceus. The evident antibody titres were detected obviously in the twice vaccinated fish 4 weeks after immunization, in which the injection group revealed higher antibody titres than the immersion group did, whereas the unvaccinated group did not showed evident change in the antibody tirtres. The vaccinated fish also showed higher relative percent survival (RPS) against bacteria than the unvaccinated fish did, in which the RPS of the injected group was higher than that of the immersion group. ECV, as well as CEV and LPS showed efficient protections in the injection and the immersion route, respectively. Results revealed that the protection of CEV against Vibrio anguillarum M3 was conspicuous.

关 键 词：牙鲆 鳗弧菌 疫苗 鳍条出血 

分 类 号：S941.42[农业科学—水产养殖] S943.399[农业科学—水产科学]