Effect of K^+ Nutrition on Growth and Activity of Leaf Tonoplast V-H^+-ATPase and V-H^+-PPase of Suaeda salsa Under NaCl Stress  

K^+营养对NaCl胁迫下盐地碱蓬生长及叶片液泡膜V-H^+-ATPase和V-H^+-PPase活性的影响(英文)

作　　者：李平华[1] 陈敏[1] 王宝山[1] 

机构地区：[1]山东师范大学生命科学学院,济南250014

出　　处：《Acta Botanica Sinica》2002年第4期433-440,共8页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金资助项目 ( 30 0 70 0 6 9);国家重点基础研究发展规划项目 (G19990 1170 0 ) ;山东省自然科学基金资助项目(Z2 0 0 0D0 1,Q99D11)~~

摘　　要：Suaeda salsa L. seedlings grown in Hoagland nutrient solution were treated with different concentrations of NaCl combined with two levels of K + (12 μmol/L and 6 mmol/L) to study the K + nutrition effect on plant growth and leaf tonoplast V-H +-ATPase and V-H +-PPase activity. Increase of K + supply in the culture solution markedly increased the fresh weight, dry weight and K + content of S. salsa plants. Western blot analysis showed that the leaf V-H +-ATPase of S. salsa was at least composed of A,B,C,D,E and c subunits, and their expression decreased with the increase of NaCl concentration under K + starvation (12 μmol/L K +), but increased under normal K + application (6 mmol/L K +). Leaf V-H +-PPase molecular weight was about 72.6 kD and its expression increased as NaCl concentration increased under both high or low levels of K + concentration in nutrient solution. There was a positive correlation between of V-H +-ATPase or V-H +-PPase activity and the amounts of their expression. Results in this study suggest that K + nutrition plays an important role in the salt tolerance of S. salsa, and K + is involved in the regulation of V-H +-ATPase or V-H +-PPase activity under salt stress.对溶液培养的盐地碱蓬 (SuaedasalsaL .)幼苗进行不同浓度NaCl胁迫并改变培养液中K+ 浓度 ,以了解K+营养对NaCl胁迫下盐地碱蓬幼苗生长及叶片液泡膜V_H+ _ATPase、V_H+ _PPase活性的影响。提高培养液K+ 浓度可明显增加盐胁迫下碱蓬植株的鲜重、干重 ,促进盐地碱蓬叶片及根部组织K+ 积累。盐地碱蓬叶片液泡膜V_H+ _ATPase至少由A、B、C、D、E及c亚基组成 ,其表达量在缺K+ 处理 (12 μmol/LK+ )下随盐胁迫浓度的增加而减小 ,而在正常K+ (6mmol/L)培养下则随盐胁迫浓度的增加而增加 ;盐地碱蓬叶片液泡膜V_H+ _PPase分子量为 72kD ,在缺K+ 和正常K+ 供应情况下 ,V_H+ _PPase均有较高表达。V_H+ _ATPase及V_H+ _PPase活性变化与其亚基表达量变化基本成正相关。结果表明 :K+ 对盐生植物碱蓬的耐盐性有重要作用 ,盐胁迫下 ,K+ 可能参与了V_H+ _ATPase和V_H+

关 键 词：Suaeda salsa GROWTH V-H  +-ATPase V-H  +-PPase K  + nutrition 

分 类 号：Q945.78[生物学—植物学]